摘要
目的探讨对低浓度乙型肝炎(下称乙肝)病毒表面抗体(抗-HBs)定性与定量检测方法的差异。方法采用酶免疫测定(EIA)一步法、时间分辨荧光法(TRF)、Axsym雅培试剂来测试抗-HBs。结果采用EIA一步法对低浓度抗-HBs标本进行测定显示,阴性37例,阳性21例;再用TRF法进行复查,阴性标本37例中有3例呈弱阳性,阳性标本21例中有2例呈阴性;以上5例结果不一致标本,采用美国Abboutt公司生产的Axsym雅培试剂检测显示,与TRF法一致。结论标志物提倡使用EIA二步法酶联试剂及定量方法,改善定性和定量检测结果差异性问题,最小限度避免结果上的差异,减少医患之间矛盾。
Objective To investigate the difference on results of qualitation and quantitation measurement of hepatic B surface antibody with low concentration. Methods Enzyme immunoassay （ELA） one-step method, timeresolved fluoroimmunoassay （TRF） and Axsym agent method were used to measure hepatic B virus antibody （HB- sAg）. Results 37 cases were HBsAg negative and 21 were positive with EIA one-step method, while 3 out of 37 negative cases were found weakly positive and 2 out of 21 positive cases were found negative with TRF redetermina- tion. The result acquired by the Axsym agent method was consistent to that by TRF. Conclusion Two-step enzyme- linked agent and quantitation method should he applied to reducing the difference between qualitation and quantitation measurement and to improving relationship between doctors and patients.
出处
《检验医学与临床》
CAS
2007年第6期485-485,487,共2页
Laboratory Medicine and Clinic
关键词
乙型肝炎病毒表面抗体
时间分辨荧光法
酶免疫测定
hepatic B virus surface antibody
time-resolved fluoroimmunoassay
enzyme immunoassay
