摘要
目的:建立HPLC法测定维生素B2的含量方法。方法:色谱柱为Agilent ZORBAX SB-C18(150mm×4.6mm,3.5μm);流动相为甲醇-0.35%磷酸的0.01mol/L庚烷磺酸钠溶液(26:74);检测波长267nm;流速1.0ml/min;柱温35℃。结果:维生素B2在1.5～12μg/ml范围内线性关系良好,回归方程为Y=94.61765X-0.46768(r=0.99995,n=6),低、中、高三种浓度的回收率分别为99.48%、99.26%、100.54%,RSD分别为0.37%、0.18%、0.18%。结论:该法快速、准确,灵敏度高,重现性好,可用于维生素B2的质量控制。
Objective： To establish a HPLC method for the determination of content of vitamin B2 in vitamin B2 bulk drug. Methods： The Agilent ZORBAX SB-C18（150 mm×4.6 mm,3.5 μm）was used, mobile phase was methanol-0.35%phosphoric acid and 0.01 mol/L sodium heptanesulfonate solution （26：74） with the flow rate of 1.0 ml/min, detected wavelength was set at 267 nm, column temperature was 35℃. Results： The calibration curve was linear at the range of 1.5-12 μg/ml for vitamin B2. The linear equation was Y=94.617 65X-0.467 68 （r=0.999 95, n=6）, The average recovery of vitamin B2 in the low, medium and high concentrations were 99.48%, 99.26% and 100.54%, respectively; RSD were 0.37%, 0.18%, 0.18%, respectively. Conclusion： This method is rapid, accurate, high sensitivity and good reproducibility, it can be used for quality control of vitamin B2 bulk drug.
出处
《中国医药导报》
CAS
2009年第1期45-47,共3页
China Medical Herald
