摘要
为探讨磷脂酶Ａ２（ＰＬＡ２）及其抑制剂溴苯酰基溴化物（ＢＰＢ）在急性坏死性胰腺炎（ＡＮＰ）中的作用机理，采用切开十二指肠行胆胰管插管注入牛磺胆酸钠的方法诱导大鼠ＡＮＰ，检测胰腺微循环血流量变化、血ＰＬＡ２、血小板激活因子（ＰＡＦ）和血栓素Ｂ２（ＴＸＢ２）水平。进行基于光镜观察的组织学评分和腺泡细胞的超微病理检查。结果显示：急性胰腺炎（ＡＰ）组在诱导后胰腺血流立即剧烈下降，此后的３小时内轻度回升，３小时后持续下降至实验结束。随着病理改变持续加重，ＡＰ组血ＰＬＡ２，ＰＡＦ和ＴＸＢ２水平持续升高。超微结构所见以粗面内质网扩张、酶原颗粒增加为特征。ＢＰＢ治疗组的胰腺血流下降轻于ＡＰ组，与ＡＰ组相比较，其组织学评分表示的病理损害显著减轻，腺泡细胞粗面内质网扩张较轻，酶原颗粒较少。ＢＰＢ组的血ＰＬＡ２，ＰＡＦ和ＴＸＢ２水平显著低于ＡＰ组。结果表明：ＰＬＡ２在ＡＮＰ的发病中起重要作用。应用ＰＬＡ２抑制剂可能是治疗ＡＮＰ的一个有效的新方法。
To study the role of phospholipase A2 (PLA2) in the pathophysiology of acute necrotizing pancreatitis (ANP), especially the relationship between PLA2 and pancreatic microcirculation, as well as the effects of PLA2 inhibitor bromophenacyl bromide (BPB) on ANP, the present paper reported the resuts of an experimental study on this topic in rats. ANP was induced by sodium taurocholate injected through duodenotomy and biliopancreatic duct catheterization in SD rats. Changes in pancreatic microcirculatory blood flow and blood levels of PLA2 and PAF were observed. Histological score of pancrease based on microscopic changes was evaluated and ultrastructural observation by electroscopy was carried out. The results showed that the pancreatic blood flow in acute pancreatitis (AP) group dramatically decreased immediatly after ANP induction, then slightly returned within 3 hours and again decreased steadily thereafter till the end of experiment. Levels of blood PLA2 and PAF rose stably as the pathological changes worsened gradually in AP group. The ultrastructural changes were characterized by enlarged rough endoplasmic reticulum (RER) and increased zymogen granules. In BPB treated group, the pancreatic blood flow decreased less dramatically than that in AP group; pathological damages were also significantly milder than that in AP group. It is concluded that PLA2 plays an important role in the pathogenesis of ANP and that the administration of PLA2 inhibitor may provide a new effective method in the treatment of ANP.
出处
《中国普通外科杂志》
CAS
CSCD
1998年第1期32-35,共4页
China Journal of General Surgery
关键词
胰腺炎
磷酰酶A2
血小板激活因子
血栓素B2
Acute pancreatitis Phospholipase A2 Platelet activating factor Thromboxane B2 Disease models,animal
