摘要
本文采用人精子染色体制备技术，分析了两名６０Ｃｏ事故性全身受照者照后６，７年的精子染色体畸变和用摸拟两受照者睾丸剂量离体照射后的健康人精子染色畸变。结果显示：在相同剂量点，离体照射组染色体畸变率显著高于全身受照组（Ｐ＜００１）；两受照组均以断裂型畸变为主，但全身受照组重接型畸变的比例显著增高；全身受照组Ｙ－精子与Ｘ－精子的比例显著增高（１６０∶１）。提示：生殖细胞在不同的发育阶段的遗传危害与对射线的敏感性是两个不同的概念，精子染色体重接型畸变的检测对评价电离辐射效应具有一定意义。两受照组Ｙ－精子与Ｘ－精子之比的差异提示辐射引进Ｘ连锁显性致死性突变的可能性。
Chromosome aberrations were analyzed with the interspecific in vitro fertilization system between human spermatozoa and zonafree hamster oocytes. The study was divided into three groups: control, wholebody irradiation was taken from two individuals had exposed to 60 Co 6 or 7 year ago; and in vitro irradiation with model at same doses. The freqqency of sperm chromosome aberrations in the group of in vitro radiation was much higher than that one of wholebody radiation with the same dose (P<0.01). Most of the structural abnormalities of chromosome observed in both radiation groups were breakage. The proportion of rejoiningtype aberrations to breakagetype ones in wholebody irradiation group was significantly higher than that in vitro one. The rate of Ysperm to Xsperm in wholebody group was significantly higher than that in control and in vitro one. The results indicated that the genetic harm in different stages of spermatogenesis and their irradiation sensitivity were two different concepts. The analysis of rejoining type aberration in human sperm may be especially important for assessing genetic effects of radiation. The difference between the proportion of YSperm to Xsperm in wholebody radiation group suggest that XLinkage dominant Lethal mutations may be induced.
出处
《癌变·畸变·突变》
CAS
CSCD
1998年第1期31-34,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
钴60
事故性全身照射
离体照射
精子
染色体畸变
60 Co wholebody irradiaton
model dose
in vitro irradiation
human sperm chromosome aberration.
