摘要
目的完善兔可控性急性心肌缺血再灌注（I／R）动物模型的制备，提高模型成功率和动物成活率。方法成年健康家兔60只，随机分为假手术组30只（Sham组）、I／R1组15只和L／R2组15只。Sham组仅穿线不接扎，I／R1组选择左冠状动脉前降支，L／R2组选择冠状动脉左缘支。开胸后以5-0 prolene线穿过冠状动脉，硅胶管圈套5-0 prolene线，用血管钳收紧硅胶管阻断冠状动脉血流，制备兔可控性心肌缺血再灌注模型。四肢安装肢体导联，通过心电图ST段的改变作为心肌缺血的评定指标。结果Sham组30只动物手术前后心电图均无变化；I／R1组结扎后，13只动物ST段无明显变化；2只动物ST段轻度压低，再灌注时无心律失常表现；L／R2组15只动物结扎后ST段均抬高明显，并渐与QRS波融合，QRS波幅随着时间的延长逐渐变小，再灌注时11只出现室性早搏，4只出现室性心动过速。结论兔可控性心肌缺血再灌注模型应选择冠状动脉左缘支。用血管钳收紧硅胶管圈套5-0 prolene线的方法可以准确地复制兔心肌缺血再灌注的实验模型，简化实验操作步骤。
Objective To establish a novel and simple method for establishing rabbit model of acute myocardial ischemia-reperfusion （MI/R）. Methods Rabbits were randomly assigned to 3 experimental groups：Sham-operated control （threading a needle without ligation）, I/R1 （anterior descending branch of coronary ligation followed by reperfusion）, I/R2 （left marginal branch of coronary ligation followed by reperfusion）. ECG was observed. Results All ST segments were elevated in I/R2 （n = 15）. The models were developed successfully. Conclusion A novel model was simple and reliable for MI/R study in the rabbit with left marginal branch of coronary ligation.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2009年第1期118-119,共2页
Chinese Journal of Experimental Surgery
关键词
心肌缺血
再灌注
模型
动物
兔
Myocardial ischemia
Reperfusion
Model,animal
Rabbit
