摘要
目的观察1，25-（OH）2D3在大鼠肾移植急性排斥反应中的作用，探讨其在免疫调节中的机制。方法行Wistar为供体，SD大鼠为受体的肾移植术，24只SD大鼠随机分为4组，检测血清中尿素氮（BUN）、肌酐（Cr）、Ca^2＋、P^3＋、白细胞介素（IL）-2、干扰素（IFN）-γ的水平及移植肾的病理变化。结果I组大鼠肾移植后5d血清中BUN、Cr、IL-2、IFN-γ水平分别明显高于Ⅱ、Ⅲ、Ⅳ组（P〈0．05），Ⅱ、Ⅲ组之间比较差异无统计学意义（P〉0．05），但分别与Ⅳ组比较均明显增高（P〈0．05）。各组Ca^2＋、P^3＋比较差异无统计学意义（P〉0．05）。病理结果显示，I组大鼠移植后肾脏排斥反应最重，其余组与之比较均有所减轻，以Ⅳ组最明显。结论1，25-（OH）2D3通过减少IL-2、IFN-γ的产生，减少排斥反应的发生，改善移植肾的功能。
Objective To study the effects of 1,25-dihydroxyvitamin D3 （ 1,25-（OH） 2 D3 ） on acute rejection of rat renal transplantation and the mechanism of its immunoloregulation. Methods Kidney transplantation from Wistar rats to SD rats was used as a model of study. Twenty-four SD rats were divided into 4 groups randomly. The levels of BUN,Cr, Ca^2＋ ,p^3＋, IL-2, and IFN-γ/ in serum were determined. HE staining inspected all specimens of kidney grafts. Results The levels of BUN, Cr, IL-2, and IFN-γ in the group Ⅰ were lower than those in groups Ⅱ , Ⅲ, Ⅳ （ P 〈 0. 05 ） , and there was no significant difference in BUN, Cr, IL-2, and IFN-γ between groups Ⅱ and Ⅲ（ P 〉 0.05 ）, but they were lower in groups Ⅱ and Ⅲ than in group Ⅳ （ P 〈 0.05 ）. The levels of Ca^2 ＋ and p^3 ＋ in serum had no significant difference among groups. The obvious histological change of rejection in grafts were observeed in group Ⅰ , and that was alleviated in the other groups, especially in group Ⅳ. Conclusion 1,25- （OH） 2 D3 reduced the acute rejection by decreasing the production of IL-2 and IFN-γ, and ameliorated the function of kidney grafts.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2009年第1期96-97,共2页
Chinese Journal of Experimental Surgery
关键词
肾移植
急性排斥反应
免疫调节
Kidney transplantation
Aacute rejection
Immunoloregulation
