摘要
目的：研究重组人粒细胞巨噬细胞集落刺激因子（ｒｈＧＭＣＳＦ）在恒河猴体内的药物动力学．方法：用酶连接免疫吸附测定法检测血浆中ｒｈＧＭＣＳＦ的含量．结果：ｉｖｒｈＧＭＣＳＦ后血药浓度时间曲线符合三房室模型．第１，２和３相的Ｔ１２分别为００５－００７ｈ，０１４－０５８ｈ和１４－４１ｈ．ＡＵＣ随剂量成比例增加．ｉｖ高剂量和低剂量的Ｃｌ和Ｋ１０都相似．ｓｃｒｈＧＭＣＳＦ后血药浓度的峰值为０９３±０１６μｇ·Ｌ－１，达峰时间为２６５±０１４ｈ，生物利用度为０６１．结论：恒河猴ｒｈＧＭＣＳＦ药物动力学数据为临床试验提供有用参考．
AIM: To examine the pharmacokinetics of iv and sc recombinant human granulocyte macrophage colony stimulating factor (rhGM CSF) in Macaca mulatta . METHODS: Plasma levels of rhGM CSF were detected with sandwich enzyme linked immunosorbent assay. RESULTS: Plasma concentration time curves after iv rhGM CSF in monkeys were best fitted with 3 compartment model. The 1st, 2nd, and 3rd phase T 12 were 0 05-0 07, 0 14-0 58, and 1 4-4 1 h. Cl and K 10 were similar between different doses, respectively. C max was 0 93±0 16 μg·L -1 , T max was 2 65±0 14 h, and elimination T 12 was 2 5±0 3 h after sc rhGM CSF. The bioavailability after sc rhGM CSF was 0 61. CONCLUSION: Pharmacokinetics of rhGM CSF in Macaca mulatta provided a useful index for clinical trial.
出处
《中国药理学报》
CSCD
1998年第1期50-53,共4页
Acta Pharmacologica Sinica
关键词
药物动力学
ELISA
RHGM-CSF
recombinant granulocyte macrophage colony stimulating factor
enzyme linked immuno-sorbent assay
pharmacokinetics
Macaca mulatta
