摘要
目的:本研究旨在探索紫杉醇、顺铂诱导卵巢癌细胞凋亡后能否具有较强的免疫原性,并可以被抗原提呈细胞交叉提呈并促进免疫应答。方法:用巨噬细胞-集落刺激因子(GM-CSF)和白介素-4(IL-4)刺激人外周血单核细胞分化诱导为树突状细胞(DC),6d后将DC和经紫杉醇联合顺铂在体外诱导发生凋亡的人卵巢癌细胞株共同培养,并以冻融细胞共培养DC组和单独DC组作对照,共培养4h后,进行激光扫描共聚焦显微镜观察DC细胞对凋亡细胞的吞噬作用,同时以流式细胞术(FCM)检测不同培养组DC的分化和成熟程度。结果:紫杉醇、顺铂诱导的凋亡卵巢癌细胞可被DC有效吞噬,与对照组相比,凋亡组DCs的表达及成熟度均明显增高,并具有统计学意义(P(0.05)。结论:紫杉醇、顺铂诱导的凋亡卵巢癌细胞具有较强的免疫原性,可以促进DC的分化和成熟。
AIM： To explore wheather apoptotic ovarian cancer cells induced by paclitaxel-cisplatin could be crosspresented by antigen presenting cells and promote immune responses. METHODS： DCs were generated from peripheral blood monocytes in RPMI1640 supplemented with GMCSF and IL-4. After 6 days＇ incubation, DCs were further co-cultured with either apoptotic HO8910 cell lines induced by paclitaxel-cisplatin or control cells for four hours. Maturation of DCs was compared among different groups according to their surface markers through flow cytometry assay and their phagocytosis ability was evaluated by confocal microscopy scanning assay. RESULTS： Apoptotic ovarian cancer cells induced by paclitaxel-cisplatincan were phagocytized more efficiently by DCs. Resulting is more cellularity and maturation of DCs in apoptotic cell-induced groups than control group （ P 〈 0.05 ）. CONCLUSION： Apoptotic ovarian cancer cells induced by paclitaxel-cisplatin exhibit strong immunogenicity, which in turn promote the maturation and antigen presentation of DCs.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2009年第1期38-41,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
上海市教育委员会科研项目(061012)
关键词
紫杉醇
顺铂
树突状细胞
细胞凋亡
抗原提呈
paclitaxel
cisplatin
dendritic cells
apoptoticcells
antigen presentation
