摘要
目的:观察酪氨酸激酶抑制剂A771726对白细胞介素13(IL-13)促成纤维细胞胶原合成作用的影响。方法:MTT法观察不同浓度的A771726对成纤维细胞的增殖作用的影响。成纤维细胞分为实验组和实验对照组,实验对照组加入IL-13(100μg/L),实验组加入A771726(50μmol/L)和IL-13(100μg/L),作用24、48、72h后,羟脯氨酸(Hyp)检测A771726对IL-13促进成纤维细胞分泌胶原蛋白的影响,RT-PCR观察A771726对IL-13促进成纤维细胞I型胶原α1基因mRNA水平表达的影响,Western blot观察A771726对IL-13促进成纤维细胞分泌I型胶原蛋白的影响。结果:A771726可抑制成纤维细胞的增殖。羟脯氨酸检测实验组48h组和72h组成纤维细胞分泌胶原蛋白的含量显著低于实验对照组(P<0.05),RT-PCR观察到实验组48h组和72h组成纤维细胞I型胶原α1基因(COL1A1)mRNA表达水平显著低于实验对照组(P<0.05),Western blot观察到实验组48h组和72h组成纤维细胞分泌I型胶原蛋白的水平显著低于空白对照组(P<0.05)。结论:酪氨酸酶抑制剂A771726阻断IL-13对成纤维细胞的促胶原蛋白合成作用,阻断IL-13促成纤维细胞I型胶原α1基因mRNA水平和蛋白水平的表达。
AIM： To observe the inhibitory effects of tyrosine kinases inhibitor A77 1726 on collagen generation induced by IL-13 in fibroblasts. METHODS： The inhibition rate of fibroblast proliferation with different concentration of A77 1726 was observed by MTT method. The fibroblasts were divided into the experimental group（AT7 1726.50 μmol/L and IL-13 100 μg/L） and the control group（ IL-13 100 μg/L）. After 2,4, 48 and 72 hours, the inhibitory effects of A77 1726 on collagen secretion of fibroblasts investigated by hydroxyproline release assay. The inhibitory effects of A77 1726 on collagen type I α1 gene mRNA expression in fibroblasts were examinated by RT-PCR. The influence of A77 1726 on collagen type I synthesis in fibroblasts was analyzed by Western blot. RESULTS： The proliferation of fibroblasts was inhibited by A77 1726. Total collagen generation was down-regulated after 48 h and 72 h stimulation of A77 1726 （ P 〈 0.05）. The expression level of collagen type I cd gene mRNA was obviously lower in the experimental group than in the control group after 48 h and 72 h stimulation of A77 1726 （ P 〈 0.05 ）. Collagen type I production of fibroblasts treated with A77 1726 for 48 and 72 hours was decreased obviously in the experimental group than in the control group （ P 〈 0.05 ）. CONCLUSION： Tyrosine kinases inhibitor A77 1726 has an inhibitory effect on collagen protein synthesis in fibroblasts induced by IL-13.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2009年第1期16-19,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
江西省卫生厅科研项目(20050455
20062016)
江西省教育厅科研项目(GJJ08083
赣教技[2007]81号)
