摘要
AIM： To study the effects of extract from Ginkgo biloba （EGb） containing 22% flavonoid and 5% terpenoid on chronic liver injury and liver fibrosis of rats induced by carbon tetrachloride （CCh）. METHODS： All rats were randomly divided into control group, CCl4-treated group, colchicine-treated group and EGb-protected group. Chronic liver injury was induced in experimental groups by subcutaneous injection of CCh and fed with chows premixed with 79.5% corn powder, 20% lard and 0.5% cholesterol （v/v）. EGb-protected group was treated with EGb （0.5 g/kg body weight per day） for 7 wk. At the end of wk 8, all the rats were killed. Liver function, liver fibrosis, oxidative stress and expression of transforming growth factor β1 （TGF-β1） a-smooth muscle actin （α-SMA） and type I collagens in liver were determined. In addition, pathology changes of liver tissue were observed under light microscope. RESULTS： The levels of alanine aminotransferase （ALT）, aspartate aminotransferase （AST） and albumin （AIb） in EGb-protected group were notably improved as compared with the CCL4-treated group （P 〈 0.01）. The contents of serum hyaluronic acid （HA）, type III procollagen （PCⅢ）, type IV collagen （CIV） and the expression of hepatic tissue TGF-β1, α-SMA and type I collagen in EGb-protected group were significantly lower than those in CCL4-treated groups （P 〈 0.05, P 〈 0.01）. The degrees of liver fibrosis in EGb-protected groups were lower than those in CCL4-treated groups （6.58 ±1.25 vs 9.52 ± 2.06, P 〈 0.05）. Compared to the CCL4-treated group, the levels of plasma glutathoine peroxidase （Se-GSH-Px）, superoxide dismutase （SOD） and malondialdehyde （MDA） were strikingly improved also in EGb-protected group （P 〈 0.05, P 〈 0.01）. CONCLUSION： EGb resists oxidative stress and thereby reduces chronic liver injury and liver fibrosis in rats with liver injury induced by CCl4.
瞄准：为了从包含 22% 的白果树 biloba (EGb ) 学习摘录的效果，长期的肝损伤和老鼠的肝纤维变性上的黄酮类和 5% terpenoid 由四氯化碳(CCl (4 )) 导致了。方法：所有老鼠随机被划分成控制组， CCl (4 ) 对待组，对待用番红花作原料而制成的植物盐基的组和保护 EGb 的组。长期的肝损伤被 CCl (4 ) 和美联储的皮下注射与 79.5% 玉米粉末， 20% 猪油和 0.5% 胆固醇(v/v ) 与食物 premixed 在试验性的组导致。保护 EGb 的组与 EGb 被对待(0.5 g/kg 体重每天) 为 7 wk。在 wk 的结束 8，所有老鼠被打死。转变生长因素 beta1 (TGF-beta1 ) 的肝功能，肝纤维变性，氧化压力和表示，一光滑的肌肉肌动朊(alpha-SMA ) 和类型我在肝的骨胶原被决定。另外，肝织物的病理变化在轻显微镜下面被观察。结果：丙氨酸 aminotransferase (中高音) 的层次， aspartate aminotransferase (著名计算机生产厂商) 和在保护 EGb 的组的白朊(白长袍的) 显著地作为与对待的 CCl (4 ) 相比被改进组(P < 0.01 ) 。浆液透明质酸的内容(哈)，类型 III 职业人员骨胶原(一种总线标准)，类型 IV 骨胶原( CIV )和肝的织物 TGF-beta1 的表示， alpha-SMA 和类型我在 保护EGb 的组的骨胶原比那些在 CCl ( 4 )对待组显著地低( P < 0.05 ， P < 0.01 )。在保护 EGb 的组的肝纤维变性的度比那些在 CCl (4 ) 对待组低(6.58 +/- 1.25 对 9.52 +/- 2.06， P < 0.05 ) 。把组，血浆 glutathoine 过氧化物酶(Se-GSH-Px ) 的层次，超级氧化物歧化酶(草皮) 和 malondialdehyde (MDA ) 比作对待的 CCl (4 ) 惊人地在保护 EGb 的组也被改进(P < 0.05， P < 0.01 ) 。结论：EGb 抵抗氧化应力并且从而在老鼠减少长期的肝损伤和肝纤维变性，肝损伤由 CCl (4 ) 导致了。
