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成年大鼠脑出血后室管膜下区、血肿周围神经前体细胞增殖研究 被引量：1

Proliferation of neural progenitor cells in the subventricular zone and around the hematoma after intracerebral hemorrhage in adult rat
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摘要 目的研究成年大鼠脑出血后室管膜下区、血肿周围组织神经前体细胞增殖的规律。方法立体定向注射胶原酶建立大鼠纹状体脑出血模型。脉冲法或连续法腹腔注射Brdu标记增殖细胞。在脑出血后第2、7、14及28天处死大鼠,行Brdu免疫组化染色,对室管膜下区、血肿周围的Brdu免疫阳性细胞作细胞记数。结果脉冲法Brdu标记,大鼠脑出血后第2天双侧室管膜下区和血肿周围Brdu阳性细胞数增加,第7天时达高峰,第14天仍可见较多的增殖细胞;连续法Brdu标记,在脑出血后第14天时室管膜下区和血肿周围可见较多Brdu阳性细胞,第28天时室管膜下区Brdu阳性细胞数降至对照水平,但血肿周围仍可见较多的Brdu阳性细胞。结论成年大鼠脑出血后可诱导双侧室管膜下区和血肿周围神经前体细胞增殖增加;室管膜下区增殖细胞可能向血肿周围迁移。 Objective To examine the proliferation of the neural progenitor cells in the subventricular zone （SVZ） and around the hematoma after intracerebral hemorrhage （ICH） in adult rats. Methods ICH was induced by stereotactic injection of type VII collagenase into the corpus striatum of adult rats, followed by pulse or continuous intrapenitoneal injection of brornodeoxyuridine （Brdu） to label the proliferating cells. The rats were sacrificed on days 2, 7, 14 and 28 following the ICH for immtmohistoehemistry of the tissues in the SVZ and around the hemotoma to determine the number of Brduimmunoreactive cells. Results With pulse Brdu labeling, a significant increase in the number of Brdu-immunoreactive cells in the ipsilateral and contralateral tissues in the SVZ and around the hematoma was observed 2-14 days, and the cell number reached the maximum on day 7 after ICH as compared with that of the sham-operated group. With continuous Brdu injection, the increase was observed on day 14 after ICH, and till day 28, the Brdu-immunoreactive cells in the SVZ decreased to the control level, but some positive cells still persisted in the tissues around the hematoma. Conclusion ICH induces transient and regional increase in the cell proliferation in the ipilateral and contraletral SVZ and tissues around the hematoma, and the proliferating cells in the SVZ may migrate towards the hematoma area.
作者 孙建军 刘勇 张蓬勃 陈新林 郭振宇 张建水 杨蓬勃 师蔚
机构地区 西安交通大学医学院第二附属医院神经外科 西安交通大学神经生物中心 西安交通大学医学院第二附属医院麻醉科
出处 《南方医科大学学报》 CAS CSCD 北大核心 2008年第11期2022-2025,2029,共5页 Journal of Southern Medical University
关键词 脑出血 细胞增殖 室管膜下区 intracerebral hemorrhage proliferation subventricular zone
分类号 R743.34 [医药卫生—神经病学与精神病学]
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参考文献19

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二级参考文献12

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南方医科大学学报
2008年 第11期

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