摘要
目的观察大鼠非酒精脂肪性肝炎(nonalcoholic steatohepattiis,NASH)组织病理形态学改变,探讨其与细胞色素P4502E1的表达和氧化应激的关系。方法用高脂饮食制备非酒精脂肪性肝人鼠模型,正常组普通饲料喂养,12w末处死各组大鼠,全自动生化仪测定血清ALT、AST、血糖、胰岛素,光镜下观察肝组织病理形态学改变,测定肝组织CYP450及同工酶CYP2E1含量,TBA法和黄嘌呤氧化酶法测定肝组织MDA含量和SOD活性。结果与对照组比较,模型组大鼠血清ALT、AST、血糖、胰岛素显著升高(P<0.05),肝组织MDA及CYP2E1含量显著增高(P<0.05),SOD活性显著降低(P<0.05),肝脏的脂肪变性程度和炎症活动度均显著增高(P<0.05)。结论成功复制NASH大鼠模型,CYP2E1与非酒精性脂肪性肝炎有关,MDA和SOD在NASH脂质过氧化反应中发挥重要作用。
Objetive To observe the pathological change of nonalcoholic steatohepatitis （NASH） and investigate its relationship between cytoehrome CYP2E1 and Oxidative stress in nonalcoholic steatohepatitis （NASH） rats. Methods To duplicate nonalcoholic steatohepafitis（NASH） rat models by high fat diet and control group received ordinary diet. The rats in both groups were killed by the end of 12 week. The serum levels of alanine aminotransferase （ALT） , aspartate transaminase （AST） , blood glucose and insulin were detected by automatic biochemistry analyzer. Liver tissue were obtained for detection of malondisldehyde （ MDA ） contents, superoxide dismutase （SOD）activity ,the contents of cytochrome P450 and CYP2E1. The pathological changes in the liver were observed by light microscopy of HE-stained section. Results Comparing with control groups, the serum levels of ALT, AST, blood glucose and insulin, the liver tissue contents of MDA and eytoehrome P450 and CYP2E1 in model groups were significantly elevated, anti-oxide SOD were decreased significantly in model group（ P 〈 0.05 ）. The seerity degree of hepatic fatty degeneration aggravaed and inflammation score in model groups were significantly increased（ P 〈 0. 05 ）. Conclusion We succeeded duplication Nonalcoholic steatohepatitis rat models can be made by high fat diet. CYP2E1 were related to the pathological change of NASH in rats. The MDA contents and SOD activity play an important role in the process of lipid peroxidation.
出处
《肝胆外科杂志》
2008年第6期457-460,共4页
Journal of Hepatobiliary Surgery
