摘要
分别使用AB-8大孔树脂、C18填料柱,从癞葡萄醇提取物中分离纯化癞葡萄皂苷,并用高效液相色谱法测定所得化合物的相对含量,最后使用质谱、核磁共振的方法对其分子结构进行了鉴定。结果成功分离纯化出一种皂苷,经测定,该皂苷的纯度为95.70%,结构鉴定为葫芦烷-5-烯-3β,22,23,24,25-四羟基-3-O-3--βD-吡喃葡萄糖(1→6)-D-吡喃葡萄糖苷。
To separate, purify and identify saponins from Momordica charantia L. Var. abbreviate Ser were studied. The ethonal extract of Momordica charantia L. Var. abbreviate Ser was purified through AB-8 and C18 column. The relative content of the obtained saponin was determined by HPLC and its structure was identified by NMR. One saponin, the relative content 95.70% and the structure cucurbitane-5-en-3β-22,23,24,25-tetrahydroxyl -3-O-3-β-D- glucopyranose （ 1→6）- D-glucopyranoside was obtained succedssfully, and was same to the Momordicoside A that has been reported in peer view international journals.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2008年第6期44-47,共4页
Journal of Food Science and Biotechnology
基金
国家"十一五"科技支撑计划重点项目(20060BA027SB03)
关键词
癞葡萄
皂苷
纯化
电喷雾质谱
核磁共振
Momordica. Charantia L. Var. abbreviater Ser
saponin
purification
ESI-MS
NMR
