摘要
用自制抗ｐ１８５ＭｃＡｂ－５Ｅ１２和进口抗ｐ１８５ＭｃＡｂ（ｃ－ｎｅｕＡｂ－３）以免疫组化法对５７例原发性乳腺癌组织的ｐ１８５表达情况进行对比研究，并以ＤＣＣ法测定其雌孕激素受体水平，对其中３４例乳腺癌组织提取ＤＮＡ进行ｃ－ｅｒｂＢ－２的Ｓｏｕｔｈｅｒｎｂｌｏｔ分析。观察ｃ－ｅｒｂＢ－２癌基因扩增与蛋白表达水平及其与雌孕激素受体水平之间的相关性。结果显示免疫组化法测定ｐ１８５蛋白表达与Ｓｏｕｔｈｅｒｎｂｌｏｔ测定基因扩增的结果个例对应的阴阳性符合率为９４．１％（３２／３４），５Ｅ１２和进口ｃ－ｎｅｕＡｂ－３的个例对应阴阳性符合率为９６．５％（５５／５７），另外，ｐ１８５表达水平雌孕激素受体呈负相关。结果表明ｃ－ｅｒｂＢ－２蛋白产物ｐ１８５表达水平基本可以反映基因ｃ－ｅｒｂＢ－２扩增水平的变化；同时，也说明５Ｅ１２有良好的特异性和灵敏性，已达进口ＭｃＡｂ水平。
The expression of p185 was determined by immunohistochemical method with McAb5E12 and cneuAB3; and the levels of ER and PR were tested by DDC technic in 57 patients with primary breast cancer. The DNA of 34 cancer tissues were extracted for Southern blot assay of cerbB2. The result showed that agreement between expression of p185 and amplification of cerbB2 was found in 94.1%; and coincidence between McAb5E12 and cneuAb3 were observed in 96.5%.There was negative correlation between overexpression of p185 and the levels of ER and PR.This study indicates that McAb5E12 has the merit of satisfactory specificity and sensitivity.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
1998年第2期111-113,共3页
Chinese Journal of Clinical Oncology
基金
国家自然科学基金
北京自然科学基金
