摘要
对24份柑桔胚性愈伤组织的保存进行了初步研究。结果发现,不同种和品种的柑桔胚性愈伤组织继代周期和适宜的保存培养基有所不同。通过检查保存13年的锦橙胚性愈伤组织的染色体数,发现99%以上的细胞染色体数没有变异,通过胚状体途径再生的锦橙植株形态、倍性也没有变异。
The preliminary study on preservation of 24 varieties or types of citrus embryonic calli was carried out . The results revealed that subculture period and media varies with genotypes. Chromosome count of the 13 years old callus of Jincheng sweet orange(C. sinensis Osbeck) showed that more than 99% of the checked cells were diploid, morphology and ploidy of plantlets regenerated from Jincheng sweet orange embryonic callus by embryogenesis is uniform in comparison with normal plantlets.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
1998年第1期89-92,共4页
Journal of Huazhong Agricultural University
基金
国家自然科学基金
湖北省自然科学基金