摘要
用金桔无菌苗的下胚轴、子叶、幼叶及幼根为外植体，在含不同激素的培养基上诱导愈伤组织发生，结果表明：以ＢＡ１ｍｇ／Ｌ＋ＮＡＡ０．５ｍｇ／Ｌ为最适宜培养基，下胚轴为最适宜材料；２。
Callus was induced from Hypocotyl,cotyledon,young leaf and young root of Fortunella margarir (lour.). The best medium for callus induction was MS supplemented with 6BA 1mg/l and NAA 0.5mg/l and the best explant was hypocotyl.2,4 D was ineffective for this induction.Green bud appeared in calli from all 4 kinds of explants,with the highest rate of 97% from h and the lowest rate of 3.7% from young leaf callus.Green bud 2～3cm in length developed into root system in rooting medium 15 days later,which was transplanted alive to field.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
1997年第3期344-348,共5页
Journal of Sichuan University(Natural Science Edition)
关键词
金桔
外植体
离体培养
再生植体
愈伤组织
Fortunella margarir(lour.), explants,in vitro culture,regenerated plantlet
