摘要
从鲤胸腺、头肾和血液中分离淋巴细胞，在添加不同剂量的柱状嗜纤维菌脂多糖（ＬＰＳ）的ＲＭＰＩ１６４０培养基中培养一定时间后，采用ＭＴＴ比色法测定ＬＰＳ对淋巴细胞的刺激指数（ＳＩ），比较了ＬＰＳ对不同组织中离体培养淋巴细胞的转化率。结果表明，ＬＰＳ添加量和培养时间对淋巴细胞的转化率都有明显的影响，当ＬＰＳ添加量为２０μｇ／ｍｌ，培养时间为７２ｈ，鲤各种组织中淋巴细胞的转化率达到峰值。血液中淋巴细胞转化率最高，ＳＩ达到１．７７；头肾其次，ＳＩ为１．３３；而胸腺中淋巴细胞的转化率最低，ＳＩ仅为１．０３。
Lymphocytes were separated from thymus, head kidney and blood of carp (Cyprinus carpio) and then incubatedin RMPI1640 medium added with various dosages of Cytophaga columnaris lipopolysaccharide (LPS) for a certain period of time. Stimulation indexes of lymphocytes from the issues were determined by MTT colorimetric assay to compare thetransformation rate caused by LPS of in vitro lymphocytes.The results showed that the transformation rate of lymphocytes was remarkably affected by both the additive dosage of LPS and incubation time. The transformation rate of lymphocytes from the issues of carp reached the peak at the additive dosage of 20μg/ml LPS and 72 hours of incubation. The transformation rate of lymphocytes from blood was highest, with stimulation index (SI) of 1.77, that from head kidney was power, with SI of 1.33, and that from thymus was lowest, with SI of only 1.03.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
1998年第1期66-70,共5页
Journal of Huazhong Agricultural University
基金
国家自然科学基金
湖北省自然科学基金
关键词
鲤鱼
脂多糖
淋巴细胞
转化率
免疫学
carp, lipopolysaccharide, lymphocyte,transformation rate
