摘要
应用毛细管电泳技术，建立了快速测定单胺氧化酶（MAO）活性的方法。研究对分离缓冲液pH值、浓度、毛细管表面改性剂十四烷基三甲基溴化铵（TTAB）浓度等影响因素进行优化，探讨了方法的可行性，确立了最佳分离条件。以70cm×50μm（i．d．）未涂敷熔融石英毛细管为色谱分离柱，运行电压15kv，运行缓冲液：0．5mmol／LTTAB，磷酸盐缓冲液（50mmol／L，pH 10．5）；紫外检测波长：214nm。MAO催化犬尿胺（Kyn）反应的产物4-羟基喹啉（4-HQ）的浓度和峰面积的线性范围为5～1000μmol／L，相关系数为0．9997，相对标准偏差（RSD）小于5．6％（n=5），检出限为2μmol／L（S／N=3）。实验证明，此方法可以用于生物样品中MAO催化活性的检测。
A method was developed for the rapid determination of monoamine oxidase （MA0） by capillary electrophoresis. The effect factors including the pH of running buffer, the concentration of electroosmotic flow modifier, the concentration of running buffer＇ were investigated. The analysis was performed in an uncoated fused-silica capillary with 50 μm id and a total length of 70 cm （40 cm to the detector） under the applied voltage of 15 kV with a running buffer of 50 mmol/L phosphate buffer saline （PBS） and 0.5 mmol/L tetradecyhrimethyl ammonium bromide （TFAB） （pH 10.5, adjusted with 1 mol/L NaOH）, detected by UV detector at 214 nm. The linear range between response and 4-hydroxyquinoline concentration, which is the product of the MAO cactalyzed reaction, was from 5μmol/L to 1 mmol/L, with correlation coefficient of 0. 9997. The precision of the method was 5.6% （n = 5 ）. The limit of detection was 2 μmol/L（S/N = 3 ）. The analysis can be finished within 10 min. This method can be used to detect the MAO activity in biological specimen.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2008年第12期1711-1715,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金重点资助项目(No.20435020)
国家"十一五"科技支撑项目(No.2006BAK03A07)
关键词
毛细管电泳
单胺氧化酶
电渗流改性剂
Capillary electrophoresis, monoamine oxidase, electroosmotic flow modifier
