摘要
RACK1是一种多功能支架蛋白,广泛参与植物生长发育过程的调节。利用反义RNA技术抑制水稻(Oryza sativa)RACK1基因的表达,分析了RACK1基因在响应干旱胁迫中的功能。采用实时定量PCR对获得的转基因植株的RACK1基因表达进行分析,结果表明转基因水稻RACK1基因表达受抑制程度达到50%左右。与非转基因水稻(对照)相比,转基因水稻耐干旱能力强,膜脂过氧化程度低且丙二醛的含量少,SOD活性高。这些结果表明,RACK1蛋白负调节水稻对干旱胁迫的耐受过程,并且这种调节作用在很大程度上与植株体内的氧化还原系统有关。
RACK1 is a scaffold protein with versatile functions and plays important roles in the regulation of plant growth and development. Transgenic rice （Oryza sativa）, with the expression of OsRACK1 anti-sense construct, was used to study possible functions of OsRACK1 in response of rice to drought stress. Real-time PCR analysis showed that the expression of OsRACK1 in transgenic rice plants was reduced by more than 50%. The tolerance to drought stress was stronger in transgenic than in nontransgenic rice plants. The epioxidation of membrane and production of malonaldehyde were significantly lower and the superoxide dismutase （SOD） activity was significantly higher in transgenic plants than in non-transgenic plants. OsRACK1 may negatively regulates tolerance of rice to drought stress, which is associated with the redox system in rice.
出处
《植物学通报》
CSCD
北大核心
2008年第6期648-655,共8页
Chinese Bulletin of Botany
基金
国家自然科学基金(No.30571120)
863项目(No.2008AA10Z120)
教育部博士点基金
