摘要
目的探讨泛耐药铜绿假单胞菌(PDRPA)氨基糖苷类耐药机制。方法应用聚合酶链反应(PCR)检测16S rRNA甲基化酶基因、氨基糖苷类修饰酶基因。结果33株PDRPA 16S rRNA甲基化酶rmtB阳性14株(42.4%);氨基糖苷类修饰酶aac(3)-Ⅱ阳性17株(51.5%)、aac(6′)-Ⅰb阳性14株(42.4%)、aac(6′)-Ⅱ阳性19株(57.6%)、ant(3″)-Ⅰ阳性16株(48.5%)、ant(2″)-Ⅰ阳性21株(63.6%),共有32株查出氨基糖苷类修饰酶基因;1株PDRPA氨基糖苷类修饰酶基因阴性者查出16S rRNA甲基化酶rmtB基因。结论PDRPA氨基糖苷类耐药机制与产16S rRNA甲基化酶和产氨基糖苷类修饰酶相关。
OBJECTIVE To investigate mechanisms of aminoglycoside resistance in pan-drug resistant Pseudomonas aeruginosa （PDRPA）. METHODS The 11 genes of 16S rRNA methylase and aminoglycoside-modifying enzymes were detected by polymerase chain reaction（PCR） and verified by DNA sequencing. RESULTS In 33 strains of PDRPA, the positive rates of rmtB,aac（3）- Ⅱ ,aac（6＇）- Ⅰ b,aac（6＇）-Ⅱ ,ant（3＂）- Ⅰ and ant（2＂）-Ⅰ were 42.4% , 51.5%, 42.4%, 57.6%, 48.5% and 63.6%. A total of 32 strains identified aminoglycoside modified genes, one strain aminoglycoside-modifying enzyme gene did not discovered, but rmtB positive. CONCLUSIONS The aminoglycoside resistance mechanisms of the PDRPA are the production of 16S rRNA methylase and aminoglycoside- modifying enzymes related.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2008年第12期1656-1658,共3页
Chinese Journal of Nosocomiology
关键词
泛耐药铜绿假单胞菌
耐药机制
pan-drug resistant Pseudomonas aeruginosa
Resistance mechanism
