摘要
目的了解鲍氏不动杆菌(ABA)耐药基因和整合子、Tn21/Tn501转座子存在状况及菌株亲缘性。方法对分离自临床27株ABA进行耐药基因、整合子、转座子检测,检测结果作聚类分析。结果27株中blaTEM、blaOXA-23群、blaADC、aac(3)-Ⅰ、aac(6′)-Ⅰ、ant(3″)-Ⅰ、qacE△1-sulⅠ、intⅠ1阳性率分别为81.5%、44.4%、85.2%、85.2%、66.7%、81.5%、85.2%、85.2%,其余基因均阴性,聚类分析显示存在克隆传播现象。结论ABA可导致克隆传播医院感染,并存在暴发性流行。
OBJECTIVE To analyze the existence state of drug-resistant gene, integron and Tn21/Tn501 transposon and the relationship of Acinetobacter baurnannii in Ningbo No. 2 Hospital by genetic mark. METHODS Twenty seven strains of A. baumannii were clinically isolated. Drug-resistant gene, integron, and Tn21/Tn501 transposon were analyzed using polymerase chain reaction（PCR）. The relationship was analyzed with cluster analysis methods. RESULTS The positive rates of blaTEM, blaOXA-23 cluster, blaADC, aac（3）-Ⅰ , aac（6′）-Ⅰ , ant（3″）-Ⅰ , qacEAl-sulⅠ, and intⅠ 1 were 81.5%, 44.4%, 85.2%, 85.2%, 66.7%, 81.5%, 85.2%, and 85.2%, respectively. The others were negative. The result of multi-gene cluster analysis showed that the clone transmission existed. CONCLUSIONS The clone transmission of A. baumannii exists in Ningbo No. 2 Hospital. The outbreak of A. baurnannii may be possible because there are more than 3 clone transmission strains.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2007年第10期1181-1184,共4页
Chinese Journal of Nosocomiology
基金
宁波市卫生局科研基金项目(2002053)
关键词
鲍氏不动杆菌
耐药基因
亲缘性
Acinetobacter baurnannii
Drug-resistant gene
Relationship
