摘要
目的：研究和血生络方对大鼠脑缺血再灌注损伤的保护作用，并探讨其对血管新生的作用。方法：将Wistar大鼠随机分为正常组、模型组、和血生络方组，后两组制备大鼠大脑中动脉缺血再灌注（MCAO／IR）模型，和血生络方组给予和血生络方灌胃，各组分别于1d、3d、7d、14d、21d时免疫组化法检测血管内皮生长因子（VEGF）和血管生成素-1（Ang-1）的表达。结果：模型组各时间点VEGF的表达较正常组增加（P〈0．05），在7d达到高峰，和血生络方组比模型组增加更明显，差异有显著性（P〈0．05～0．01）。模型组各时间点Ang-1的表达较正常组增加（P〈0．05），14d达到高峰，和血生络方组比模型组增加更明显，差异有显著性（P〈0．05～0．01）。结论：和血生络方可能通过增强VEGF、Ang—1的表达来促进大鼠脑缺血再灌注后的血管新生。
Obiective ： To study the protective effect and the angiogenesis mechanism of Hexueshengluofang on focal cerebral ischemia/reperfusion in rats. Methods： The rats were randomly divided into normal group, model group and Hexueshengluofang group, after MCAO/IR （Middle Cerebral Artery Occulusion/ Reperfusion Injury） models were set up in latter two groups,the Hexueshengluofang group were given Hexueshengluofang, expression of VEGF and Ang-1 was detected with immunonhistochemical method after lday,3days, 7days, 14days. Results：The exression of VEGF in the Hexueshengluofang group were reached a peak on the 7th day, and significantly increased as compared with those in the model group and the normal group at each time（ P 〈 0.05-0.01）. The exression of Ang-1 in the Hexueshengluofang group were reached a peak on the 14th day, and significantly increased as compared with those in the model group and the normal group at each time（ P 〈 0.05-0.01 ）. Conclusion： Hexueshengluofang＇s improving ischemic stroke is carried out possibly through up regulating the expression of VEGF and Ang-1 in the cerebral tissue.
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2008年第11期840-842,共3页
JOURNAL OF BASIC CHINESE MEDICINE
