摘要
采用KromasilC18不锈钢柱(150mm×4.6mmi.d.,5μm),以甲醇-水混合溶剂(体积比95∶5)为流动相,流速1.0mL/min,光电二极管阵列检测器,检测波长254nm,柱温35℃,建立了水体和茶汤中硫氟肟醚残留的高效液相色谱分析方法。硫氟肟醚水样直接用二氯甲烷进行液-液分配萃取,茶汤采用正己烷-丙酮(体积比1∶1)萃取。在0.01～5.0mg/L质量浓度范围内,硫氟肟醚峰面积与质量浓度呈现良好的相关性。当井水、池塘水、河水和茶汤中添加质量浓度为0.01～1.00mg/L硫氟肟醚时,其平均回收率分别为92.59%～100.10%、94.77%～100.68%、91.87%～101.06%、88.94%～99.46%,变异系数分别为0.98%～2.32%、1.46%～3.09%、2.45%～3.94%、1.40%～3.97%。
A quantitative high performance liquid chromatography method has been developed for the determination of HNPC-A2005 residues in the environmental aquatic system using photodiode array detector. HNPC-A2005 was extracted by liquid-liquid partition from water samples into dichloromethane, and from tea soup samples into acetonehexane （1：1, by vol）. The analysis was performed on a Kromasil Ctsstainless steel column （150mm×4.6mmi.d.,5μm） with mixture of methanol-water（95：5, by vol） as the mobile phase at a flow rate of 1.0 mL/min, at the column temperature of 35 ℃ and the ultraviolet detection wavelength 254 nm. The calibration curve for HNPC-A2005 was in good linearity within the concentration range of 0.010-5.0 mg/L. The average recovery of five spiked concentration levels for HNPC-A2005 in well water, pond water, river water and tea soup was 92.59-100.10%, 94.77-100.68%, 91.87-101.06% and 88.94-99.46%, respectively, and its corresponding coefficient of variation （CV） was 0.98-2.32%, 1.46-3.09%, 2.45-3.94% and 1.40-3.97%.
出处
《农药》
CAS
北大核心
2008年第12期896-898,共3页
Agrochemicals
基金
国家“十一·五”科技支撑计划课题(2006BAE01A03-1)
