摘要
采用三重-比光谱导数分光光度法同时测定氨基酸注射液中的3种芳香氨基酸,讨论了测定酪氨酸、苯丙氨酸和色氨酸的实验条件。实验结果表明,在pH7.4的NaH2P04.NaOH缓冲溶液中,3种氨基酸的线性范围分别为:酪氨酸2.0×10^-6~2.8×10^-5mol/L,苯丙氨酸2.0×10^-5,1.6×10^-3mol/L.色氨酸4.0×10^-7~1.6×10^-6mol/L。回收率在95%-105%之间。
Simultaneous determination of tyrosine, phenylalanine and tryptophan by triple ratio spectra derivative spectrophotometry was discussed. The linear ranges were 2.0×10^-5,1.6×10^-3 mol/L mol/L (Tyrosine), 2.0×10^-6~2.8×10^-5mol/L (Phenylalanine), and 4.0×10^-7~1.6×10^-6 mol/L (Tryptophan). The recoveries ranged from 95% to 105 %. The method was simple, convenient and fast.
出处
《分析试验室》
CAS
CSCD
北大核心
2008年第12期88-91,共4页
Chinese Journal of Analysis Laboratory