摘要
用１４０个１０碱基随机引物对外来猪种杜洛克猪、斯格猪、长白猪、大约克夏猪及地方猪种蓝塘猪、大花白猪的基因组ＤＮＡ进行ＲＡＰＤ分析，其中２３个引物无扩增产物，６６个引物的扩增产物表现为多态，５１个引物的扩增产物表现为单态，扩增产物的电泳条带数在１～１１条之间，平均为４２条。从相似系数及聚类分析结果可以看出，斯格猪与长白猪、蓝塘猪与大花白猪的相似系数较高，亲缘关系也较近，而２个地方猪种与４个外来猪种之间则相应地较低、较远；在４个外来猪种中，除杜洛克猪外，另３个外来猪种又因曾直接或间接地引用过中国地方猪种作为育种素材，因而表现出与地方猪种相对较近的亲缘关系。
total of 140 short primers, of arbitrary nucleotide sequence,were used singly in polymerase chain reactions to amplify DNA fingerprints in pools of DNA from six pig breeds,including Duroc,Seghers,Landrace,Large White,Lantang and Dahuabai. Of these primers,23 amplified no DNA figerprint,66 produced polymorphic DNA fingerprints and 51 monomphic DNA fingerprints. Average number of bands per primer was 4.2,ranging from l to 11 bands. Similarity coefficients and cluster analysis ,calculated according to the DNA frigerprints,indicated that there were close relationships between the Lantang and Dahuabai,and between the Seghers and Landrace.Duroc expressed a farther relationship to native breeds than to other imported pig breeds
出处
《华南农业大学学报》
CAS
CSCD
北大核心
1997年第A00期85-89,100,共5页
Journal of South China Agricultural University
关键词
RAPD
猪
相似系数
亲缘关系
RAPD (Random amplified polymorphic DNA)
pig breeds
similarity cofficients
relationship
