摘要
利用简单随机抽样获得亚东山羊（ｎ＝６），高原型藏山羊（ｎ＝１５）样本，以吕梁山羊为对照进行基因组ＤＮＡＲＡＰＤ及ＲＦＬＰ标记的分析。结果表明，序列为５′－ＴＧＧＴＧ－ＣＡＣＴＣ－３′的随机引物可以作为区分亚东山羊与高原型藏山羊的标记引物，Ｓ及ＭＡＰＤ在某种程度上揭示了类群间遗传相似性；ＥｃｏＲⅠ，ＨａｅⅢ单酶切，ＥｃｏＲⅠ／ＨｉｎｄⅢ双酶切亚东山羊和高原型藏山羊基因组ＤＮＡ分别产生可观察的ＲＦＬＰ条带。
Compared with Luliang Goat (n=8),Yadong (n=6) and plateau type goats (n=15) samples were collected with simple random sampling.The genomic DNA were determined by RAPD and RFLP.The result indicated that the 14th primer could be confirmed as a marked primer to discern Yadong and plateau type goats.The genetic variation between populations was promulgated by similarity index and MAPD to some extent.Respectively,2 and 1 RFLP bands were detected with EcoRⅠ and HaeⅢ singlecutting,I and 2 bands with EcoRⅠ/HindⅢ doublecutting.
出处
《西北农业大学学报》
CSCD
北大核心
1998年第1期17-20,共4页
Journal of Northwest Sci-Tech University of Agriculture and Forestry（Natural Science Edition)
基金
国家自然科学基金
