摘要
５６周龄的母泰和鸡分为３组，第１组（ｎ＝３５）用血管活性肠肽（ＶＩＰ）类似物与牛血清白蛋白（ＢＳＡ）的偶联物进行免疫，第２组（ｎ＝３３）用矿油佐剂与水的乳化物进行注射，第３组（ｎ＝３６）作空白对照。试验结果显示：与对照组相比，免疫组日抱窝率明显减少，产蛋高峰后的产蛋率明显高于对照组，且各组间蛋的受精率和孵化率无明显差异。第１、２组血浆ＶＩＰ类似物抗体及催乳素（ＰＲＬ）水平的酶联免疫吸附试验（ＥＬＩＳＡ）检测结果显示：第１组二免后ＶＩＰ类似物抗体水平最高，Ｐ／Ｎ达４９３±１１６，而第２组的检测结果为阴性；第１组免疫后的血浆ＰＲＬ水平明显低于同期的第２组。以上结果表明，在泰和鸡中主动免疫ＶＩＰ可以降低血浆的ＰＲＬ水平，减少抱窝率，提高产蛋率。
ifty-six-week-old Tairle hens were divided into three groups. Group 1 hens (n=35) were immunized with the conjugate of C-terminate fragment of vasoactive intestinal peptide (VIP) and bovine serum albumin(BSA). Group 2 hens (n=33) were injected with the mixture of mineral oil adjuvant and water; Group 3 hens (n=36) served as nontreated controls. The results showed that, in contrast with the controls. daily broody rates of the immunized group decreased greatly, while the rates of egg production were higher than that of the control after the peak of egg production. Fertilizing rates and incubating rates of eggs had no remarkable difference amomg groups. Antibody to the fragment of VIP and prolactin in plasma of group 1 and 2 were detected with enzyme-linked immunosorbent assay (ELISA). The antibody in group 1 reached peak value (P/N was 4.93±1.16) two weeks after the second immunization, But it wasn't detected in group 2. After immunization, the prolactin level in group 1 was much lower than that in group 2. These results demonstrated that active immunization against VIP decreased plasma prolactin and broody rate, increased egg production.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
1997年第A00期45-50,共6页
Journal of South China Agricultural University
关键词
血管活性肠肽
抱窝
产蛋
催乳素
免疫
泰和鸡
VIP
broodiness
egg production
prolactin
immunization
TaiHe hens
