摘要
目的:探讨V-ATPase在非小细胞肺癌(NSCLC)中的表达及其在病理分类、分级中的差异性;检测相应癌组织对化疗药物的敏感性,分析其与V-ATPase表达的相关性,为进一步研究V-ATPase耐药性及其机制提供依据。方法:采用免疫组化EnVinsion法、免疫荧光法检测V-ATPase在92例NSCLC中的表达,激光共聚焦观察其定位,统计学分析差异性;用MTT法检测相应癌组织对化疗药物的敏感性,分析其与V-ATPase表达的相关性。结果:V-ATPase阳性表达主要定位于胞膜上和胞质中,鳞癌阳性率71.43%、腺癌阳性率83.72%,两者表达差异性非常显著(P=0.000);鳞癌Ⅱ阳性率58.33%、Ⅱ-Ⅲ阳性率84.00%,差异性显著(P=0.014);腺癌中高分化阳性率76.7%、低分化为100.0%,差异性显著(P=0.012)。环磷酰氨、吉西他滨、阿霉素、紫杉醇、顺铂化疗药在NSCLC组织中药物敏感性试验结果与其相应组织V-ATPase表达相关性检验,P值均<0.05,相关系数rs分别为-0.697、-0.654、-0.598、-0.216、-0.604,其中鳞癌中rs分别是-0.584、-0.512、-0.544、-0.269、-0.306,腺癌分别为-0.742、-0.607、-0.63、-0.349、-0.707。结论:V-ATPase在非小细胞肺癌中高表达,表达的高低与病理分类、分级有关,并很可能与NSCLC化疗药物耐药有关。
Objective： To investigate the expression of vacuolar-H ＋-ATPase （V-ATPase） protein in non-small cell lung cancer （NSCLC）, to analyze the differences in V-ATPase expression among different pathological classifications and grades, and to explore the correlation of V-ATPase expression with the chemoresistance of NSCLC. Methods： We used immunohistochemistry and immunofluorescence to detect the expression of V-AT- Pase in 92 cases of NSCLC. The location of the protein was observed under light microscope and confocal laser scanning microscope. The chemoresistance of these cases was assayed by MTT method, and the corre- lation between the chemoresistance and V-ATPase expression in NSCLC were analyzed by statistics. Results： The V-ATPase protein was overexpressed at a rate of 71.43% in squamous cell lung cancer and 83.72% in lung adenocarcinoma, with a significant difference （P=0.000）. In squamous cell lung cancer, the positive expression rates of pathological grade Ⅱ and Ⅱ-Ⅲ were 58.33% and 84.0%, respectively, with a statistical significance （P=0.014） The positive expression rates of moderately differentiated and low differentiated lung adenocarcinoma were 76.70% and 100.0%, respectively, with a significant difference （P=0.012）. The P values of the correlation tests between the chemosensitivity of cyclophamide, gemcitabine, adriamycin, paclitaxel, or cisplatin and V-ATPase expression in the NSCLC were all less than 0.05. The correlation coefficients（rs） were-0.714,-0.687,-0.612,-0.33, and-0.67, respectively. Conclusion： The V-ATPase protein is overex- pressed in NSCLC at a higher expression rate in lung adenocarcinoma than in the squamous cell lung cancer. The chemoresistance of NSCLC is probably correlated with the V-ATPase expression.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2008年第22期1294-1298,共5页
Chinese Journal of Clinical Oncology
基金
陕西省科研攻关基金资助(编号:2004K13-G7)
