摘要
用二色桌片参（Mensamariaintercedens）酶解液处理转化的人胚肺成纤维细胞（NLF）后，细胞生长受到显著抑制，抑制率达64．84％，光镜下细胞透光性加强，铺展较好，细胞核形更为规则，核畸形现象减少；线粒体结构趋于正常；软琼脂集落实验表明，细胞集落形成率由23ｘ10－5下降至12．5ｘ10－5，裸小鼠致瘤实验显示抑瘤率为82．39％，差异显著（p＜5％）。结果提示，二色桌片参酶解液可能逆转转化的HLF细胞的恶性表型，对其有一定的诱导分化作用。
The transformed Human Lung Fibroblast(HLF) cell line was used to study the anti-tumor effects of Mensamaria intercedens (Lampart). The results indicate that this sea cucumber enzyme-digested solution has effects on this cell line.The growth inhibition percentage of the treated HLF cell was 64. 84%. The cell became more transparent and better spread. The nucleus was oval with clear and continuous nuclear membrane. The colony efficiency in soft agar decreased from 23 ｘ10-5to 12. 5 ｘ10-5. The tumor weight caused in the nude mice decreased by 82. 39%, with a significant difference (p<5% ). All these suggest that the enzyme-digested Mensamaria intercede ns solution has the inhibitiong effect on the cancerous phenotype and can somewhat induce the differentiation of the cell line. However, whether it affects other cancer cell lines and its mechanism should be further studied.
出处
《台湾海峡》
CAS
CSCD
1997年第3期270-274,T001,共6页
Journal of Oceanography In Taiwan Strait
基金
厦门市1994年海洋科技项目
关键词
二色桌片参
转化细胞
抗癌效应
酶解液
海参
Mensamaria intercedens, Transformed cell, Human Lung Fibroblast, Anticancer effects
