摘要
【目的】本实验旨在探讨异氟醚麻醉对大鼠海马乙酰胆碱(ACh)释放昼夜节律的影响,并比较在暗期及明期麻醉对该节律变化的影响。【方法】采用活体微透析技术,从大鼠海马处连续收集透析液3d,并自动注射到高效液相色谱仪中以测定ACh浓度。大鼠共分3组(每组6只):对照组;明期给药组(从11:00至15:00时予1.4%异氟醚麻醉);暗期给药组(从23:00至03:00予1.4%异氟醚)。所有大鼠放置于明暗周期下喂养(明期07:00-19:00)。【结果】对照组海马ACh呈明显的昼夜节律变化,暗期ACh平均值较明期明显增高(173.4%,185.3%vs.107.4%,104.0%,P<0.01);明期给药组,麻醉使第一个明期ACh均值减少(94.0%vs.107.4%,P<0.05);麻醉后ACh在暗期及明期的浓度与对照组相比,无显著差异(183.2%vs.185.3%,101.4%vs.104.0%,P>0.05)。暗期给药组,麻醉使第一个暗期ACh均值明显减少(136.0%vs.173.4%,P<0.01);而且麻醉后明期的ACh均值较对照组明显增高(125.4%vs.107.4%,P<0.01),麻醉后暗期ACh均值较对照组明显减低(154.2%vs.185.3%,P<0.01)。【结论】在暗期给予异氟醚麻醉较在明期麻醉对海马ACh的昼夜节律影响明显。
[ Objective] To investigate the effect of isoflurane anesthesia on the circadian rhythm of hippocampal extracellular acetylcholine （ACh） level in rats and compare this effect of isoflurane anesthesia administered during the light phase and during the dark phase. [Methods] Using in vivo microdialysis technique, the dialysate was collected from the hippocampus for 3 d, and was auto-injected into the high performance liquid chromatography, by which ACh concentration was measured. The rats were divided into three groups （n = 6 in each group）： Group Control; Group Light （1.4% isoflurane was administered from 11：00 to 15：00）; Group Dark （1.4% isoflurane was administered from 23：00 to 03：00）. All the rats were housed under a light/dark cycle （light phase 07：00-19：00）. [Results] The hippocampal ACh release showed a clear daily rhythm in Group Control： the mean ACh in the dark phases was higher than those in the light phases （173.4%, 185.3% vs 107.4%, 104.0%, P 〈 0.01）. For Group Light, as compared with Group Control, anesthesia made the mean ACh in the first light phase reduce （94.0% vs. 107.4%, P 〈 0.05） ; the mean ACh in the dark phase and light phase after anesthesia did not differ from their correspondents of Group Control （183.2% vs.185.3%, 101.4% vs.104.0%, P 〉 0.05）. For Group Dark, anesthesia made the mean ACh in the first dark phase reduce greatly （136.0% vs. 173.4%, P 〈 0.01）; after anesthesia, compared with Group Control, the mean ACh was increased in the following light phase （125.4% vs. 107.4%, P 〈0.01）, and decreased in the dark phase （154.2% vs. 185.3%, P 〈 0.01）. [Conclusion]The influence of isoflurane anesthesia on the hippocampal ACh circadian rhythm was pronounced when administered during the dark phase than those administered during the light phase.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2008年第6期758-761,781,共5页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省医学科研基金(A2008221)
关键词
乙酰胆碱
海马
昼夜节律
微透析
麻醉
acetyleholine
hippocampus
circadian rhythm
microdialysis
anesthesia
