摘要
目的:建立固相萃取浓缩-高效液相色谱-串联质谱法测定化妆品中10种糖皮质激素类药物的方法。方法:样品经提取后,通过Oasis HLB固相萃取柱进行浓缩净化,用高效液相色谱-串联质谱法测定(以正离子电喷雾电离,检测方式为多级反应离子监测(MRM)),内标法定量。结果:糖皮质激素在5～150ng/mL范围内,线性相关系数均>0.99;在5～40μg/kg添加水平,10种糖皮质激素的回收率为52.5%～112.5%,相对标准偏差(RSD)为3.21%～11.08%(n=6)。结论:该方法具有快速、定量准确、检测灵敏等优点,适于化妆品中多种糖皮质激素药物的测定。
Objective： To establish a method for the determination of eleven glucocorticoids （prednisolone, methylprednisolone, prednisone, dexamethasone, triamcinolone, betamethasone, triamcinolone acetonide, fludrocortisone acetate, beclomethasone, cortisone and flumethasone） in cosmetics by solid phase extraction and HPLC-MS/MS. Methods： Various cosmetic samples were extracted with methanol under ultrasonication. The extract was centrifuged and the upper solution was HLB solid phase extraction cartridge. The analytes were determined b electrospray ionization and under multi-stage reaction mode （MRM）. 150ng/mL （ r 〉 0.99 ）. The recoveries for 11 glucocorticoids spiked 112.5% （ r〉 0.99 ）, the RSDs were between 3.21% and 11.08% （ simple, sensitive and accurate. concentrated and purified by Oasis y HPLC-MS/MS using positive ion Results： The linear range was 5 in cosmetics ranged from 52.5 % to n = 6 ） Conclusion. The method is
出处
《分析仪器》
CAS
2008年第6期23-28,共6页
Analytical Instrumentation
基金
上海出入境检验检疫局科研经费资助项目(HK007-2007)
