摘要
发酵过程中控制菌体为适宜的菌球形态对于丝状真菌发酵的工业化放大具有重要的研究意义。本文针对米根霉在5L发酵罐上种子培养过程中的形态学控制进行了研究,确定了最适宜形成菌球的培养条件分别为:控制发酵罐内的孢子浓度在5×104～8.3×104/mL的范围内,搅拌转速350r/min,种子液pH值稳定在2.80,以20g/L的葡萄糖浓度作为最佳种龄的经验值确定种子培养时间为52h。该条件下可以获得表面光滑的直径约为1mm的结实菌体。发酵结果表明,相比于絮状形态,菌球形态更有利于富马酸的生成。
It is of great significance to grow the strain into the appropriate pellet form for the industrial fermentation of filamentous fungi. The morphology control in the seed culture process of Rhizopus oryzae in the 5L stirring bioreactor was investigated. The optimal conditions for the pellet formation were as follows： the spore concentration in the fermentor should be controlled in the range of （5×10^4） ~ （8.3×10^4） /mL, the best agitation speed was 350r/min, the best pH in the medium was maintained at 2.80, and by using residual glucose concentration of 20g/L as an empirical value the best seed age was determined at 52h. In this way, the smooth compact pellets with the diameter around lmm were obtained. Fermentation results showed that the pellet morphology was more beneficial to the fumaric acid production than the filamentous morphology.
出处
《化工进展》
EI
CAS
CSCD
北大核心
2008年第12期1967-1971,共5页
Chemical Industry and Engineering Progress
基金
国家自然科学基金(20576054)
国家863计划(2006AA02Z240)
国家973计划(2007CB707805)资助项目
关键词
米根霉
形态控制
菌球
发酵罐
富马酸
Rhizopus oryzae
morphology control
pellet
fermentor
fumaric acid
