摘要
本文通过采用基本培养基、诱导培养基和植物激素试验等探索利用菽北种茶外殖体进行快速组织培养的最适培养基。研究发现,用菽北种茶带腋芽的茎段做为外殖体进行组织培养可获得再生植株,并可建立快速无性繁殖系。实验表明,芽增殖最适培养基为1/2MS+BA1.0mg/L+IBA0.2mg/L;生根壮苗最适培养基为1/4MS+BA0.5mg/L+IBA0.1mg/L+Al3+0.01mg/L。此外,还探讨了菽北种茶组织培养中褐变问题以及试管苗的移栽。
The clonal propagation of Yabukita is developed through culture of stem with lateral bud.In using MS basal medium with addition of different content ration of plant hormones. The results show that the best proliferation medium is 1/2MS ＋ BA1.0mg/L ＋ IBA0.2mg/L;Induction of root is better for shoots in 1/4MS ＋ BA0.5mg/L ＋ IBA0.1mg/L ＋ A13＋0.01mg/L.
出处
《安徽农学通报》
2008年第22期101-102,共2页
Anhui Agricultural Science Bulletin
