摘要
Objective： To evaluate the effects oftramadol on the proinflammatory responses in a rat model of incisional pain by investigating its effects on nociceptive thresholds and serum interleukin-6 （IL-6） and IL-2 levels. Methods： Forty-two male Sprague-Dawley （SD） rats scheduled for plantar incision were randomly divided into 7 groups 01=6 in each group）. Rats in Group 1 receiving general anesthesia with no incision were served as control; At 30 min before skin incision, Groups 2-5 were given 5 ml normal saline or 1, 10, and 20 mg/kg tramadol, respectively, intraperitoneally （i.p.）; Group 6 received 10 mg/kg tramadol after operation; Group 7 received 10 mg/kg tramadol before incision, followed by 200 μg/kg naloxone after operation. Mechanical allodynia was measured by electronic yon Frey filament to evaluate the nociceptive thresholds 1 h before incision, and 1 h and 2 h after operation. Serum IL-6 and IL-2 levels were measured by enzyme-linked immunosorbent assay （ELISA） 2 h after operation. Results： Mechanical thresholds decreased significantly and serum IL-6 level increased significantly after operation in Group 2 compared with control （P〈0.01）, and these changes were reversed respectively by tramadol in a dose-dependent manner （P〈0.05 and P〈0.01, respectively）. IL-2 level remained unchanged after operation in Group 2, but decreased in Group 3 （P〈0.05）, then gradually returned to the normal level in Groups 4 and 5. The intraperitoneally injected tramadol （10 and 20 mg/kg） produced a potent and dose-dependent antinocicptive effect on the lesioned paw. The antinocicptive effects of tramadol were partially an- tagonized by naloxone （200 μg/kg）, suggesting an additional non-opioid mechanism. Conclusion： The results suggest that tramadol could be a good choice for the treatment of pain under the conditions that immunosuppression may be particularly contraindicated.
目的将在在一只老鼠的煽动性的回答由在 nociceptive 阀值和浆液 interleukin-6 和 IL-2 层次(IL-6 ) 上调查它的效果 incisional 疼痛建模的职业人员上评估 tramadol 的效果。42 只男 Sprague-Dawley (SD ) 老鼠为脚底的切口安排了的方法随机被划分成 7 个组(在每个组的 n=6 ) 。在没有切口的组 1 收到的一般麻醉的老鼠作为控制被服务；在在皮肤切口前的 30 min，组织 2 ∼5 分别地， intraperitoneally 被给盐的 5 ml 正常或 1， 10，和 20 mg/kg tramadol (i.p ) ；组 6 在操作以后收到了 10 mg/kg tramadol；组 7 在切口前收到了 10 mg/kg tramadol，在操作以后由 200 μg/kg naloxone 列在后面。机械 allodynia 被电子 von 司丰饶细丝测量评估 nociceptive 阀值在切口前的 1 h 和在操作以后的 2 h。浆液 IL-6 和 IL-2 层次被连接酶的免疫吸着剂试金(ELISA ) 测量在操作以后的 2 h。结果机械阀值显著地减少了，浆液 IL-6 水平在与控制(P<0.01 ) 相比的组 2 在操作以后显著地增加了，并且这些变化被 tramadol 以一种剂量依赖者方式分别地颠倒(P<0.05 和 P<0.01，分别地) 。在在组 2 的操作以后未改变的仍然是，但是在组 3 减少了的 IL-2 水平(P<0.05 ) ，然后逐渐地在组回到了正常水平 4 和 5。intraperitoneally 注射的 tramadol (10 和 20 mg/kg ) 在 lesioned 爪上生产了一个有势力和剂量依赖者 antinocicptive 效果。tramadol 的 antinocicptive 效果被 naloxone (200 μg/kg ) 部分反对，建议另外的 non-opioid 机制。结果建议那 tramadol 的结论能是为在免疫力的抑制可以特别地被禁忌的条件下面的疼痛的治疗的一种好选择。
基金
Project (No.2006A031) supported by the Medical Foundation Insurance of Zhejiang Health Bureau, China
