摘要
采用肉汤微量稀释法,选用22种常用抗菌药物,对98株动物源沙门菌进行药敏试验,结果显示:菌株对磺胺类、四环素类药物及萘啶酸普遍耐药,对氨基糖苷类药物耐药率较低,对氟喹诺酮类药物高度敏感;菌株多重耐药率为67.35%(66/98)。采用PCR方法检测菌株Ⅰ类整合子的流行情况,并分析其携带的耐药基因盒。结果98株沙门菌中Ⅰ类整合子的检出率为50.0%(49/98),并且均携带耐药基因盒,基因盒以dfrA17-aadA5的组合形式最为常见;Ⅰ类整合子阳性菌株的多重耐药率为95.92%(47/49),阴性菌株的多重耐药率为38.78%(19/49)。上述结果表明Ⅰ类整合子普遍存在于兽医临床耐药沙门菌中,其流行与菌株多重耐药性具有一定的相关性。
The MICsof 22 kinds of autimierobial agents to Salmonella isolates from animals were determined by the broth microdiluaon. The results showed that these isolates were high resistant to sulfanilamide, tetracyclines and Nalidixic acid, whereas low resistant to aminoglycosides, and almost all of isolotes were sensitive to fluoroquinolones; 66 isolates （67.35 %） were multidrug resistant. PCR and DNA sequencing were used for characterization of class 1 integrons and gene cassettes. 50.0% （49/98） of the isolates were positive for the intⅠ1 gene, and the gene cassette array dfrA17-aadA5 was the most prevalent. The multidrug resistance rate was 95.92% （47/49） in intⅠ1 positive isolates, and 38.78% （19/49） in negative isolates. The results revealed that class 1 integrons were widely present in Salmonella isolates from ani-mals and were correrated well with multidrug resistance.
出处
《中国兽医杂志》
CAS
北大核心
2008年第11期6-9,共4页
Chinese Journal of Veterinary Medicine
基金
国家"十一五"科技支撑计划项目(2006BAK02A03)
关键词
沙门菌
Ⅰ类整合子
耐药基因盒
多重耐药
salmonella
class 1 integron
gene cassette
multidrug resistance
