摘要
探讨了MBR膜污染层胞外多糖的分离提取及纯化方法。结果表明,采用80℃水浴法提取物中胞外多糖含量为86.0%,粗多糖经酶解-Sevag法去除蛋白质,通过DEAE-纤维52、Sephacry-400 HR柱分离纯化得到多糖EPS-A1。紫外光谱分析多糖EPS-A1未见蛋白质与核酸的特征吸收峰,红外光谱分析其具有典型的多糖特征吸收峰。
Separation and purification of extracellular polysaccharide （EPS） from biofouling layer of MBR were investigated. The results showed that the amount of EPS extracted by thermal treatment （80 ℃, 30 min） was 86%. The protein was removed by Sevage method. The EPS was further purified by cellulose chromatography. The purified sample EPS-A1 was separated by Sephacryl S-400 HR column chromatography. Analysis of polysaccharide by UV spectrum showed that there were no absorption peaks of protein and nucleic acid at 280 nm and 260 nm. FT-IR results showed the EPS-A1 had typical absorption peaks of polysaccharide.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2008年第5期45-49,共5页
Journal of Food Science and Biotechnology
基金
国家自然科学基金项目(50678073)
