摘要
目的观察氯化镧对植入实验犬颌骨自体骨缺损区的组织工程骨成骨情况的影响。方法选成年杂种犬9只,从股骨抽取骨髓、以密度梯度离心法获取骨髓基质细胞(BMSCs)并诱导培养为成骨细胞。以5.564μg/ml的氯化镧(La3+)干预第三代BMSCs并将其与猪脱钙冻干骨(fdDBM)复合,体外培养7d后植入实验犬颌骨人造骨缺损处(2cm×1cm×0.8cm),另设BMSCs加fdDBM、fdDBM及空白对照组。3个月及6个月后处死实验犬获取颌骨标本并加工处理作形态学观察及X线骨密度分析。所得数据用SAS6.12软件包进行方差分析。结果BMSCs与fdDBM复合回植3个月后实验犬骨缺损能完全或大体为新生骨修复。La3+干预组回植区新生骨的密度值较高,但与对照组的密度值比较差异无统计学意义(P>0.05)。回植6个月后BMSCs加fdDBM与单纯fdDBM回植区新生骨的骨密度值比较差异无统计学意义(P>0.05),但该二组的骨密度值明显高于对照组(P<0.05)。结论5.564μg/ml浓度的氯化镧对回植入实验犬人造颌骨缺损处的组织工程骨成骨作用无明显负面影响。
Objective To observe the effects of lanthanum chloride（La^3＋） on the osteogenesis of tissue-engineering bone implanted into the autologous bony defects in dog jaws. Methods Bone marrow stromal cells （BMSCs） from femoral bone marrow of 9 dogs were separated by density gradient centrifugation, cultured in vitro and induced to be osteoblast. The third generation BMSCs were interfered with La^3＋ of 5.564 μg/ml and combined with the pig freeze-dried demineralize bone matrix （fdDBM）, incubated in vitro for 7 days and then grafted into the autologous bony defects （2 cm × 1 cm x 0.8 cm） of dog jaws. BMSCs-fdDBM, unseeded fdDBM and empty defects served as control groups. At 3 and 6 monthes after operation,the dogs were sacrificed and the implanted site of the jaws were harvested. The specimens were processed for morphological observation and radiographic bone-density analysis. The data was analyzed by SAS 6.12 software for ANOVA. Results New osteoid structure could be observed through histological exam of all the specimen of all groups. The density values of new bone in the implanting area treated with La^3＋ was higher but there was no obvious statistic significance as compared with that of the control groups. 6 monthes later, the density values of new bone in BMSCs-fdDBM group and fdDBM group had no obvious difference （P 〉 0.05） but were significantly higher than that of empty control groups （P 〈 0.05）. Conclusion La^3＋ at the concentation of 5.564 μg/ml has no obvious negative effect on osteogenesis of tissue engineering bone.
出处
《中华口腔医学研究杂志（电子版）》
CAS
2008年第4期5-8,共4页
Chinese Journal of Stomatological Research(Electronic Edition)
基金
上海市自然科学基金资助项目(04ZR14139)
关键词
氯化镧
骨髓基质细胞
组织工程骨
冻干脱钙骨
Bone marrow stromal cells （BMSCs）
Tissue-engineering-bone
Lanthanum chloride （La^3＋）
Freeze-dried demineralized bone matrix （fdDBM）
