摘要
作者采用新生纯系小鼠颅骨体外培养模型，综合利用钙转换率和组织形态学观察等指标，客观地评价了白细胞介素－１（ＩＬ－１）调节骨吸收的作用。结果表明，ＩＬ－１具有强烈的刺激骨吸收的活性，其两种不同形式ＩＬ－１α和ＩＬ－１β的活性接近。２００～４０００ｎｇ／ｍｌ的白细胞介素－１受体拮抗剂（ＩＬ－ｌｒａ）能完全阻断２０ｎｇ／ｍｌＩＬ－１α刺激骨吸收的作用，即使是在加入ＩＬ－１α４小时后，再加入ＩＬ－ｌｒａ同样能阻断ＩＬ－１α诱导的骨吸收作用。此外发现，ＩＬ－１在其作用最初２４小时内，未能增加培养颅骨骨吸收活性；培养７２小时后组织学观察颅骨破骨细胞数量增多。揭示ＩＬ－１刺激骨吸收的作用机制是：刺激破骨细胞前体增殖分化成熟，增加破骨细胞数量，而对成熟破骨细胞缺乏直接作用。
AbstractEffects of interleukin 1(IL-1)on the calciumturnover and histological changes were studied in thein vitro culture system of the neonatal mouse calvari-ae. Both IL-1α and IL-1β were shown to stimulate cal-cium release from the cultured calvaria. 200～4000ng/ml interleukin 1 receptor antagonist(IL-lra)blockedcompletely the effect of 20ng/ml IL-1α on the calciumrelease, Histological examination showed that IL-1 in-creased osteoclastic bone resorption in the culture cal-varia. Both IL-1α and IL-1β were shown to be stimulifor bone resorption with no difference in potency.Itwas of interest to find that IL-1 failed to increase boneresorption during its first 24 hours of incubation.These would suggest that IL-1 stimulates bone resorp-tion by increasing osteoclast number rather than theactivity of preexisting mature osteoclasts.
出处
《中华骨科杂志》
CAS
CSCD
北大核心
1995年第12期849-851,共3页
Chinese Journal of Orthopaedics
