摘要
目的：分析野生地黄群落之间的遗传差异类型，比较RAPD和ISSR分子标记在分析地黄野生种质遗传多样性中的应用。方法：应用RAPD和ISSR分子标记技术，对55份地黄材料进行了遗传差异分析。结果：平均每条RAPD引物扩增出16．00条带，平均每条ISSR引物扩增出19．08条带，多态性位点百分率分别为89．58％和94．32％；聚类分析表明，55份地黄分成7大类群。结论：野生地黄具有丰富的遗传多样性；不同地理居群的相似性为0．63—0．98；ISSR标记比RAPD标记能检测到地黄种内更高的遗传差异性。
Objective： To analyze the genetic diversity of wild Rehmannia glutinosa and evaluate and compare random amplifled polymorphic DNA （RAPD） and inter sample sequence repeat （ISSR） for analysis of R. glutinosa accessions. Method： Two molecular markers, RAPD and ISSR were used for analyzing 55 wild R. glutinosa accessions. Result： Average 16.00 and 19.08 bands were amplified by RAPD primers and ISSR primers respectively, and the percentage of polymorphic bands were 89.58% and 94.32% respectively; Fifty-five R. glutinosa accessions categorized into 7 clusters were identified by unweighted pair-group method, arithmetic average （UPGMA） method. Conclusion： A high level of genetic diversity of wild Rehmannia glutinosa was displayed at DNA level, and genetic diversity coefficient of R. glutinosa from different production areas was 0.63-0.98, and ISSR marker can detect higher ge- netic diversity of R. glutinosa germplasms than RAPD marker.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第22期2591-2595,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30472155)
北京市自然科学基金项目(5062035)
