摘要
目的评价一种自制止血海绵作为医用生物材料的安全性。方法通过体内埋植法和体外酶降解法考查其降解作用;采用MTT法评价其对鼠成纤维细胞L929的细胞毒性;采用腹腔注射法观察其全身急性毒性;采用皮肤刺激、皮内刺激、眼刺激法考察其刺激作用。结果该止血海绵具有一定的降解性,无细胞毒性和急性毒性,无刺激性。结论实验结果提示该止血海绵是一种安全的,具有潜在临床应用价值的生物医用材料。
Objective To evaluate the safety of a self-producing hemostatie sponge as a medical biomaterial. Methods The degradability of the hemostatic sponge was examined by implantation in vivo and enzymolysis in vitro respectively; its cytotoxicity on L929 mouse fibroblasts was monitored using the MTT assay; its systemic acute toxicity was observed by abdominal injection, The local irritation of the sponge was investigated by skin, subcutaneous and eye application. Results The hemostatic sponge possesses receivable degradability and has no cytotoxicity, systemic acute toxicity and irritation. Conclusion The results suggested that this hemostatic sponge might be one of promising and safe medical biomaterial with potential clinic application.
出处
《中国海洋药物》
CAS
CSCD
2008年第5期51-54,共4页
Chinese Journal of Marine Drugs
基金
军队"十一五"课题(06MA201)
关键词
止血海绵
降解性
细胞毒性
全身急性毒性
刺激作用
hemostatic sponge
degradability
eytotoxicity
systemic acute toxicity
irritation
