摘要
目的观察重组人促红细胞生成素（r—HuEPO）对大鼠颅脑损伤后细胞间黏附分子－1（ICAM－1）、IL－6和IL－10表达的影响，探讨其颅脑损伤后的抗炎症反应机制。方法75只雄性Wistar大鼠按随机数字表法分为假手术组（n=5）、颅脑损伤组（n=35）及r-HuEPO治疗组（n=35）。采用改进Feeney法制作大鼠颅脑损伤模型，应用免疫组织化学法检测伤侧脑组织ICAM—1的表达．双抗体夹心酶联免疫（ELISA）法检测血清IL-6、IL-10表达水平。结果与假手术组相比，颅脑损伤组、r-HuEPO治疗组ICAM-1、IL-6和IL-10表达水平均明显增加；其中r-HuEPO治疗组与颅脑损伤组比较，ICAM—1和IL-6的表达相对降低，IL-10的表达相对增加。结论r—HuEPO可能通过促进IL-10的表达，抑制ICAM-1、IL-6的表达而减少颅脑损伤后细胞炎症反应．发挥脑保护作用.
Objective To investigate the effect of recombinant human erythropoietin （r-HuEPO） on the expressions of intracellular adhesion molecule-1 （ICAM-1）, interleukin-6 （IL-6） and IL-10 after traumatic brain injury （TBI） in rats, and explore the anti-inflammatory mechanisms of r-HuEPO. Methods Seventy-five adult male Wistar rats were randomly divided into sham-operated group （n=5）, TBI group （n=35） and r-HuEPO treatment group（n=35）, and experimental TBI was induced according to Feeney＇s method in the latter two groups. Serum levels oflL-6 and IL-10 were determined with enzyme-linked immunosorbent assay and the expression of ICAM-1 protein was detected using immunohistochemistry after the injury. Results Compared to the sham-operated group, the expression levels oflCAM-1, IL-6 and IL-10 were significantly increased in TBI group. Application ofr-HuEPO significantly reduced the expressions oflCAM-1 and IL-6, but increased the serum level oflL-10 after the injury. Conclusion R-HuEPO may decrease the inflammatory response to brain injury by increasing the expression of IL-10 and inhibiting ICAM-1 and IL-6 expressions to provide neuronal protection after TBI.
出处
《中华神经医学杂志》
CAS
CSCD
2008年第11期1131-1134,共4页
Chinese Journal of Neuromedicine
基金
全军十一五医学基金（06G032）
