摘要
目的探讨拟胆碱药物卡巴胆碱对脂多糖(LPS)刺激后单核细胞人类白细胞组织相容性抗原-DR (HLA-DR)表达率及淋巴细胞凋亡率的影响。方法分离、培养正常人外周血单核细胞和淋巴细胞,对照组仅加1640培养液,LPS组加LPS刺激,LPS+卡巴胆碱组先用不同浓度卡巴胆碱(100、10、1、0.1、0.01μmol/L)预处理细胞后,再加入LPS(100μg/L)刺激,培养12h后用流式细胞术检测CD14^+单核细胞HLA-DR表达率和外周血淋巴细胞凋亡率。结果LPS单独刺激单核细胞时,其HLA-DR表达率明显降低,用卡巴胆碱预处理后,HLA-DR表达率随着卡巴胆碱浓度的增高而增高。LPS单独刺激淋巴细胞时,淋巴细胞凋亡率明显增加,而用卡巴胆碱预处理后,淋巴细胞凋亡率随着卡巴胆碱浓度的增高而降低。结论卡巴胆碱对LPS引起的人血单核细胞和淋巴细胞免疫功能下调有显著抑制作用。
Objective To investigate the effect of cholinergic drug carbachol on immune function of monocytes and lymphocytes in human peripheral blood stimulated by lipopolysaccharide（LPS）. Methods Monocytes and lym- phocytes were separated from peripheral blood in healthy adults. The cells for test were divided into control, LPS stimulation alone and LPS stimulation -- carbachol groups. Different doses （100, 10, 1, 0.1, 0.01μmol/L）of carbachol were added to monocytes and lymphocytes in 1 ×10^9 cells/L, followed with stimulation by LPS（100μg/L）. After cultivating for 12 h, expression of HLA-DR antigen on CD14＋ monocyte and lymphocyte apoptosis ratewere determined by direct immunofluorescence on a flow cytometer. Results HLA-DR expression was significantly lower in monocytes stimulated by LPS than those not stimulated. When pretreated with carbachol, HLA-DR expression significantly increased in a concentration-dependent manner. Apoptosis rate in lymphocytes stimulated by LPS alone was significantly higher than those in controls, and much decreased when pretreated with carbachol, which also showed a concentration-dependent manner. Conclusion The results suggested that carbachol had a significant effect on LPS induced immunosuppression in human monocytes and lymphocytes.
出处
《中华老年多器官疾病杂志》
2008年第5期365-367,共3页
Chinese Journal of Multiple Organ Diseases in the Elderly
