摘要
目的探讨隐丹参酮对孕期高雄激素血症大鼠雄性子代的雄激素合成的影响。方法Wistar雌性大鼠,在孕中期颈背部皮下注射丙酸睾丸酮,连续3天。以其所生雄鼠作为实验对象,运用放射免疫方法检测血清睾酮(T)、17-羟孕酮(17-OHP)、血糖及胰岛素水平;然后以隐丹参酮灌胃14天,检测其对雄激素水平的影响,运用免疫组织化学方法观察睾丸间质细胞17α-羟化酶的表达情况。结果孕中期雌鼠处于高雄激素状态,其所生雄鼠T值无变化,17-OHP(1.66±0.30)μg/L、空腹胰岛素水平(61.69±21.62)IU/L、胰岛素抵抗指数17.51±6.25均明显高于对照组,分别依次为(0.76±0.05)μg/L、(26.57±5.48)IU/L、7.18±0.78(P<0.05,P=0.05265,P<0.05);隐丹参酮可降低17-OHP,中药组治疗前(1.66±0.30)μg/L,治疗后为0.20±0.07)μg/L,(P<0.05);免疫组织化学结果显示,睾丸间质细胞17α-羟化酶的表达无改变。结论给予孕中期雌鼠注射丙酸睾丸酮,所生雄鼠的T水平虽无改变,但其前体合成物质17-OHP水平明显升高,胰岛素敏感性降低,提示有高雄激素合成和胰岛素抵抗;而隐丹参酮能够降低17-OHP水平,而其胰岛素增敏作用不明显,说明有降低雄激素合成的作用。
Objective To study the effects of cryptotanshinone on androgen synthesis for the prenatally androgenized male rats. Methods On days 16-18 of pregnancy, rats were injected s.e. with testosterone propionas continuously for 3 days; male offspring were studied as subject. Serum concentrations of testosterone （T） , 17a- hydroxy progesterone （17-OHP）, blood glucose, and insulin were measured by radioimmunoassay. Then, the rats were treated with cryptotanshinone by garage for 14 days, and the levels of serum T, 17-OHP and insulin were detected and the 17a-hydroxylase protein expression in interstitial cell was measured using the method of immunohisto-chemistry. Results There was no difference between the male groups who were prenatally androgenized in serum levels of T, but the 17-OHP, fasting insulin levels and homeostatic model assessment for insulin resistance （HOMA-IR） elevated significantly （P 〈 0.05）. Cryptotanshinone could lower the levels of 17-OHP （ P 〈 0.05） but had no effect on 17a- hydroxylase. Conclusion Prenatally androgenized male rats exhibit elevated 17-OHP and diminished insulin sensitivity. Cryptotanshinone could decrease 17-OHP, but has no effect on insulin, indicating it may reduce androgen synthesis.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2008年第11期1001-1004,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.30572404)
黑龙江省自然科学基金重点项目(No.ZJY0506-01)
黑龙江省研究生创新科研项目(No.YJSCX2007-0180HLJ)
关键词
高雄激素
睾酮
17-羟孕酮
胰岛素
隐丹参酮
hyperandrogenemia
testosterone
17a- hydxoxy progesterone
insulin
cryptotanshinone
