摘要
测定了黑曲霉X-1菌株液体发酵制备的粗酶液中酶的种类,以及利用该粗酶液对不同植物纤维素原料、pH值、温度、底物浓度和酶解时间分别进行实验。结果表明:该粗酶液酶系组成为复合酶,除具有很高的木聚糖酶外,还含有较高的纤维素酶、β-葡聚糖酶及少量的纤维二糖酶。同时得到酶解糖化玉米芯的最佳条件:pH4.5、温度45℃,加入3%玉米芯粉酶解48h,糖化液中总糖达17.94mg/mL,还原糖达14.16mg/mL,糖化率达到59.4%。
The kinds of crude enzymes produced by Aspergillus niger X- 1 in liquid fermentation were detected. The crude enzymes were studied to different plant celluloses, with different pH, temperature, substrate concentration and enzymolysis time, respectively. The results indicated that there were several kinds of enzyme in the crude enzyme solution, which contained high content of xylanase and cellulase, some β-glycanase and a little fibre disaccharide enzyme. With the crude enzymes, the optimal enzymolysis and saccharification conditions of corncob were as follows： pH 4.0, enzymolysis temperature 45 ℃, the concentration of corncob powder 3.0%, enzymolysis time 48 h. Under above conditions, the contents of total sugar and reducing sugar in the saccharifying solution reached 17.94 mg/ml and 14.16 mg/ml, respectively. And the saceharifying efficiency was 59.4%.
出处
《中国酿造》
CAS
北大核心
2008年第11期44-46,共3页
China Brewing
基金
江西农业大学校青年教师基金重点项目(赣农大科发[2005]03)
校青年教师基金项目(1891)
关键词
黑曲霉X-1
玉米芯
酶解糖化条件
Aspergillus niger X- 1
corncob
enzymolysis and saccharification condition
