摘要
[目的]观察不同浓度的防风对正常小鼠小肠推进及胃排空的抑制作用。[方法]清洁级昆明小鼠60只,随机分为对照（NS）组和5个防风组,每组10只,NS组予0.85%NaCl,5个防风组分别以5、10、15、20、25 g/kg剂量,灌胃后30 min处死小鼠,观察各组的小肠推进率和胃残留率。[结果]防风各组均对小鼠小肠推进和胃排空有一定程度的抑制作用,其中5、101、5 g/kg剂量组小肠推进率与NS组比较差异有统计学意义（P〈0.05）,15 g/kg组作用最强;5、10、25 g/kg剂量组胃排空率与NS组比较差异有统计学意义（P〈0.05）,而15、20 g/kg剂量组与NS组比较差异无统计学意义。[结论]防风有抑制小鼠小肠推进的作用,在5-15 g/kg范围内效果随剂量的增大而增强,在〉15-25 g/kg范围内随剂量的增大而减小;防风对胃排空也有不同程度的抑制,在5-15 g/kg范围内抑制作用随剂量的增大而减小,在〉15-25 g/kg范围内随剂量的增大而增强。
[Objective]To observe the inhibition of Radix Saposhnikoviae （RS） on the small intestinal propulsion （SIP） and gastric emptying （GE） in normal mice. [Methods]Sixty mice were divided into control and five experimental groups randomly. Normal saline （NS） was used as control treatment while 5 dosages （5, 10, 15, 20, 25 g/kg） of RS were adopted for the experiment. The method for determination of phenol red was used to observe the small intestinal propulsion （SIP） and gastric emptying （GE）. Those mice were sacrificed 30 min after intragastric administration and the small intestinal propulsion ratio （SIPR） and gastric residual ratio （GRR） of each group were observed. [Results]All of different dosages of RS had inhibition effect on the SIP and GE at different degrees in normal mice. For the SIPR, the difference between low-dosage （5, 10, 15 g/kg） groups and the NS group was significant （P〈0. 05）, and the 15 g/kg group was the most powerful. As for the GRR, the difference between the NS group and the experimental groups （5, 10, 25 g/kg） was significant （P〈0. 05）, while that between the NS group and the experimental groups （15, 20 g/kg） had no statistical value. [Conclusion]RS can inhibit the intestinal propulsion. With the dose increasing from 5 to 15g/kg, the SIP enhances, but in the dose range from 1 to 25g/kg, the SIP reduces.
出处
《中国中西医结合消化杂志》
CAS
2008年第5期305-307,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
江苏省兴卫工程中医药领军人才基金(苏卫规财2007/158)
关键词
防风
小鼠
小肠推进率
胃残留率
Radix Saposhnikoviae
inhibitory function
mice
small intestinal propulsion ratio
gastric residual ratio
