摘要
目的研究祛风通络方对脂多糖刺激大鼠肾小球系膜细胞表达炎性细胞因子TGF-β1、IL-6的影响及其作用机制。方法采用体外培养大鼠系膜细胞和血清药理学方法,分为空白组,病理组,治疗组和阳性对照组。采用生物素核酸-蛋白结合凝胶迁移电泳分析法检测NF-κB的表达;RT-PCR检测TGF-β1mRNA和IL-6mRNA表达。结果祛风通络方能够抑制脂多糖刺激肾小球系膜细胞中NF-κB活化,且在祛风通络方的作用下,TGF-β1mRNA和IL-6mRNA表达均呈不同程度减弱。结论提示祛风通络方可能通过抑制NF-κB活化,下调TGF-β1mRNA和IL-6mRNA的表达达到减轻系膜细胞增殖,防止肾小球硬化的作用。这可能是祛风通络方治疗系膜增生性肾炎的机制之一。
Objective To investigate the effect of Qufeng Tongluo （QFTL） decoction on lipopolysaccharide （LPS）-induced rat mesangial cell proliferation and explore the possible mechanisms underlying the therapeutic effects. Methods Thirty-two rats were randomized into normal control, glomerulonephritis model, QFTL treatment and positive control groups, and serum samples were obtained from these groups. Rat mesangial cells with or without LPS exposure were treated with the sera, and the expression of nuclear factor-KB （NF-κB） was detected using electrophoretic mobility shift assay （EMSA）, and the expressions of transforming growth factor-β1 （TGF-β1） and interleukin-6 （IL-6） mRNAs were detected with RT-PCR. Results QFTL decoction inhibited the activation of NF-κB in LPS-stimulated rat mesangial cells stimulated by LSP, and lowered the expressions ofTGF-β1 and IL-6 mRNA. Conclusion QFTL decoction can inhibit LPS-induced rat mesangial cell proliferation by decreasing the expression of TGF-β1 and IL-6 mRNA as a result of suooression NF-KB activation.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2008年第10期1825-1827,1830,共4页
Journal of Southern Medical University
基金
陕西省科技计划项目基金(2004K16-G9)
陕西省中医管理局项目基金(2005028)
