摘要
采用微波辅助提取鱼腥草叶总黄酮,并用正交试验进行工艺参数的优化。同时选择7种大孔树脂,比较其对鱼腥草叶总黄酮的吸附量和解吸率,筛选出较优的大孔树脂并对其动态吸附及解吸性能进行考察。结果表明:优化的工艺条件为乙醇浓度50%,固液比1∶50(w/v),预浸泡30 min,微波作用时间30 s,微波功率540 W;与传统乙醇提取法相比,微波法使每次提取时间由3 h减少为30.5 min,提取率从92.14%增加到95.93%。NKA-9型大孔树脂对鱼腥草叶总黄酮有较好的吸附和解吸效果;较优的吸附分离工艺参数为:样液pH值在3.00～3.50,上样液流速1 mL/min,上样液浓度2.21～3.10 mg/mL,用70%乙醇洗脱时,解吸率达97.8%,3BV洗脱液基本上能将鱼腥草叶总黄酮洗脱下来。
The microwave-assisted extraction technique studied, and the optimization of technological parameters for flavonoids from Houttuynia cordata Thunb. leaves was were obtained by orthogonal experiments. At the same time, seven types of macroporous resin were selected to compare their performances in absorbing and desorbing flavonoids from Houttuynia cordata Thunb. leaves. The optimal macroporous resin was decided at last and its dynamic absorbing and desorbing behavior was studied. Results show that the optimum conditions are as follows： ethanol concentration is 50%, the ratio of solid to liquid is 1 ： 50（w/v）, dipping 30 min, microwave action time is 30 s and microwave power is 540 W. Compared with traditional ethanol extraction, microwave-assisted extraction is able to reduce the extraction time from 3 h to 30.5 min and increases extraction rate from 92.14% to 95.93%. And NKA-9 type macroporous resin possesses higher absorption and desorption capacity. The optimum technological parameters of adsorption and separation are pH 3.00-3.50, velocity of flow 1 mL/min and sample solution concentration 2.21-3.10 mg/mL. When 70% ethanol is used as eluant, the desorption capacity is 97.8%, and 3BV eluant can desorb flavonoids from Houttuynia cordata Thunb. leaves basically.
出处
《农业工程学报》
EI
CAS
CSCD
北大核心
2008年第10期227-232,共6页
Transactions of the Chinese Society of Agricultural Engineering
关键词
微波辅助提取
大孔树脂
吸附
分离
鱼腥草叶
黄酮
microwave-assisted extraction, macroporous resins, adsorption, separation, Houttuynia cordata Thunb. Leaves, flavonoids
