摘要
目的：探讨rhBD3-BPI是否对革兰阴性菌具有抗菌活性。方法：采用密度梯度稀释法检测rhBD3-BPI对铜绿假单胞菌标准株和不同来源的临床多药耐药革兰阴性菌株的抗菌活性，确定其最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。以基因工程重组的人β-防御素3（rhBD3）作为对照。结果：rhBD3-BPI对各待检菌的MIC在1～4μg·mL^-1之间，MBC在4～8μg·mL^-1之间。rhBD3对革兰阴性菌的MIC在4—8μg·mL^-1之间，MBC在8～16μg·mL^-1之间。rhBD3-BPI和rhBD3对G^-菌的最小抑菌浓度和最小杀菌浓度有统计学差异（分别为P=0．0060、P=0．0053），rhBD3-BPI对G^-菌的最小抑菌浓度和最小杀菌浓度低于rhBD3对G^-菌的最小抑菌浓度和最小杀菌浓度。结论：rhBD3-BPI和rhBD3均对革兰阴性细菌具有显著的抗菌活性，两者之间具有显著差异。
Objective： to investigate the bactericidal activities of recombinant fusion protein of human β-defensin 3 （hBD3） and bactericidal/permeability increasing protein （BPI） against Gram-negative bacteria. Methods： rhBD3-BPI was serially diluted and incubated with standard Pseudomonas aeruginosa or clinically-isolated gram-negative bacteria strain. Minimal inhibitory concentration （MIC） and minimal bactericidal concentration （MBC） were determined. Recombinant hBD3 served as control. Results： rhBD3-BPI had a MIC against the bacteria at 1-4 p.g ~ mL-1 , and a MBC at 4-8μg·mL^-1. As a control, rh- BD3 had a MIC at 4-8 μg·mL^-1and a MBC at 8-16 μg·mL^-1. Significantly statistical difference in the MIC and MBC of rh- BD3-BPI and rhBD3 was found against each individual gram-negative bacterial strain. Conclusion：rhBD3-BPI has bactericidal activities against gram-negative strains.
出处
《军医进修学院学报》
CAS
北大核心
2008年第5期368-370,共3页
Academic Journal of Pla Postgraduate Medical School
关键词
Β-防御素
融合蛋白
革兰阴性菌
beta-defensins
fusion protein
gram-negative bacterial strain
