摘要
目的研究鸟苷酸环化酶C（GC-C）和尾型同源盒转录因子2（CDX2）基因与蛋白在胃癌及癌前病变组织中的表达并探讨其临床意义。方法收集30例手术切除的胃癌及相应癌旁5cm胃黏膜组织，另32例非胃癌患者胃镜下取活检标本，其中23例肠上皮化生、9例异型增生。应用逆转录（RT）-PCR检测GC—C和CDX2 mRNA在胃癌及癌旁组织中的表达，Western印迹和间接免疫荧光组化技术检测GC—C和CDX2蛋白的表达，同时检测两者在肠上皮化生和异型增生中的表达。结果R-PCR显示GC-C和CDX2 mRNA在胃癌中的表达率分别为20／30和19／30，显著高于癌旁组织（0／30和0／30，P值均=0．000）。Western印迹检测GC-C和CDX2蛋白在胃癌组织中表达率分别为19／30和17／30，显著高于癌旁组织（0／30和0／30，P值均=0．000）。免疫荧光检测GC—C和CDX2在癌旁组织中不表达，在肠上皮化生组织中表达率为39．1％和39．1％、异型增生组织为55．6％和55．6％、胃癌组织为56．7％和60．0％，与癌旁组织间差异有统计学意义（P值均＝0．000）。但在肠上皮化生、异型增生和胃癌间阳性率比较差异无统计学意义（P值均〉0．05）。两者在肠型胃癌中的表达高于弥漫型（P值分别=0．009和0．024），但与年龄、性别、病灶大小、临床病理分期、分化程度和淋巴结转移等因素无关（P值均〉0．05）。在肠上皮化生和胃癌中GC-C与CDX2的表达呈正相关（r分别＝0．4524和0．3845，P分别＝0．0371和0．0408）。结论GC—C和CDX2的异常表达与胃黏膜癌变的发生有关，可能参与人胃腺癌致癌过程的调节，检测GC-C与CDX2有助于早期胃癌和胃癌前病变诊断。
Objective To investigate the expressions of guanylyl cyclase-c（GC-C） and caudal-type homeobox transcription factor 2 （CDX2） in human gastric tissues and precursor lesions and its significance. Methods The cancerous and paracancerous （5 cm from cancer lesion ）samples from 30 cases of gastric cancer and 32 samples including 23 intestinal metaplasia and 9 dysplasia were collected. The mRNA expressions of GC-C and CDX-2 were detected by reverse transcriptase-polymerase chain reaction （RT-PCR） and proteins of GC-C and CDX-2 were measured by using Western blot and immunofluorescence methods. Results The mRNA expressions of GC-C and CDX-2 were absent in paracancerous tissues, but were 66.7% and 63.3% in cancerous tissues, respectively（P=0. 000）. The Western blot indicated that expressions of GC-C and CDX-2 were 19/30 and 17/30 in cancerous tissues, but absent in paracancerous tissues（P = 0. 000）. The immunofluoreseence examination revealed that GC-C and CDX-2 expressions were 39.1% and 39.1% in intestinal metaplasia, 55.6% and 55.6% in dysplasia, and 56.7% and 60.0% in cancerous tissues, respectively, but absent in paraeancerous tissues. Moreover, expressions of GC-C and CDX-2 showed a statistical difference between intestinal-type and diffuse-type of gastric cancer （P〈 0.05） ,but had no correlation with age, sex, size of the lesion, clinical stage and lymphnode metastasis. The positive correlation was found in expressions of GC-C and CDX2 between intestinal metaplasia and cancerous tissues（r=0. 4524 and 0. 3845,P=0. 037 and 0. 0408, respectively）. Conclusions The over expressions of GC-C and CDX2 in human gastric cancer is associated with precursor lesions ana may play an important role in the carcinogenesis of gastric cancer. The examination of GC-C and CDX2 expressions will be helpful in diagnosing gastric cancer and precursor lesions.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2008年第10期673-677,共5页
Chinese Journal of Digestion
