摘要
目的利用酶法提取红树林放线菌DNA。方法利用溶菌酶破壁、蛋白酶K和SDS除蛋白,提取红树林放线菌DNA,所得DNA可直接进行16SrDNAPCR扩增检测,对放线菌进行鉴定。结果成功地从红树林土壤中提取放线菌DNA。结论本方法提取到的放线菌基因组DNA结构完整,可以直接进行PCR扩增等分子生物学操作,为高通量筛选和鉴定放线菌奠定基础。
Objective To study a brief and safe method of extracting genomic DNA from actinomycetes of mangrove forest. Methods Lysozyme was used to lyze cell wall and proteinase K,and SDS was used to remove protein,extracted genomic DNA from mangrove forest, which was studied by 16SrDNA PCR. The actinomycetes were identified by PCR. Results Genomic DNA was extracted successfully from actinomycetes isolated from soil of mangrove forest. Conclusion The DNA is suitable for PCR amplification. The method can be used in Actinomycetes systematics and rapid indentification.
出处
《广西医学》
CAS
2008年第11期1657-1659,共3页
Guangxi Medical Journal
基金
广西壮族自治区教育厅科研课题(桂教科研[2006]26号)
关键词
红树林
放线菌
基因组DNA
Mangrove forest
Actinomycetes
Genomic DNA
